Abstract P112: Vivasure: An Oxygen-Carrying Lipid Emulsion for Resuscitation
Oxygen (O2) and mediators of hemorrhagic shock are lipophilic and should be preferentially taken up by lipid micelles(LM). Also, LM are colloids that can fill intravascular space. These facts suggest that LM could be used for resuscitation. To test this we removed 55– 60% of the blood volume/2 minutes via the carotid artery from mice anesthetized with ketamine/xylazine. Vivasure(20% soybean oil micelles + 5% albumin) was compared to normal saline + 5% albumin (NSA), Ringer’s lactate + 5% albumin (RLA) and shed blood each as a volume equal to the blood removed. Blood pressure (BP) was recorded for one hour. n=6 mice for each infusate. P<0.05 for Vivasure (VS) higher than blood at 5,15, and 30 minutes as shown below for mean BP. VS was higher than the other preparations as well. Under identical conditions LM (n=6) was compared to Ringer’s lactate(n=6) at 0, 30 and 60 minutes. No albumin was present. At each time systolic and diastolic BP were greater after LM(p<0.05). Mass spectroscopic measurement of O2 content of LM after less than one minute of O2 infusion was 75.5 mg/L (n=3). O2 consumption with hemoglobin of 7.0gm/dl and mixed venous O2 of 60% is 63.9mg/L. By kinetic data all O2 on LM is available to tissues.
VS is a usable for resuscitation
VS is superior to blood and other fluids
LM O2 carrying capacity is significant Y axis = % of Pre-Hemorrhage Mean BP achieved after Infusion