Abstract 5634: Molecular Imaging of Bone Marrow Mononuclear Cell Survival and Homing in Murine Peripheral Artery Occlusive Disease
Introduction. Bone marrow mononuclear cell (BMMN) therapy has emerged as a potential treatment for peripheral artery obstructive disease (PAOD). However, much is unknown about cellular kinetics following transplantation. This study aimed to use molecular imaging to provide insight into cellular behavior following different transplantation methods.
Methods. BMMN were isolated from male F6 transgenic mice (FVB background) that express firefly luciferase (Fluc) and green fluorescence protein (GFP). BMMNs were characterized by bioluminescence imaging (BLI). Male FVB mice (n=33) underwent ligation of the superficial femoral artery and were randomized into 4 groups to receive:
Single intramuscular (i.m.) injection of 2×106 MN;
4 weekly i.m. injections of 5×105 MN;
PBS control; and
5×106 MN intravenously (i.v.).
Cellular kinetics were measured by BLI. Paw perfusion was monitored by Laser Doppler Perfusion Imaging (LDPI).
Results. Fluc expression correlated with cell number in all groups (r2=0.97). In vivo BLI revealed dismal cell survival within 4 weeks following both i.m. transplanted groups. Following i.v. transplantation, BLI showed early homing to the injured area as well as to liver, spleen, and bone marrow. LDPI showed no significant differences in paw perfusion among i.m. groups.
Conclusion. This is the first study to assess kinetics of transplanted BMMN in PAOD using in vivo molecular imaging. BMMN survival after both i.m. and i.v. transplantation is short lived and BMMN do not preserve perfusion in this model.