Abstract 5530: Associations Between Arterial Blood Pressure and Adipokines Expression in Subcutaneous, Femoral and Pericardial Adipose Tissue in Patients With Coronary Atherosclerosis
Background: Adipokines such as interleukin-6 (IL-6), monocyte chemoattractant protein-1 (MCP-1), and adiponectin are synthesized in adipose tissue (AT) and may affect the development of hypertension. We characterised the synthesis of these adipokines in subcutaneous, femoral and pericardial AT of patients with advanced atherosclerosis, and we examined their impact on arterial blood pressure.
Methods: Fifty patients undergoing elective CABG (14 hypertensives 36 non-hypertensives) were recruited. Subcutaneous AT was collected from the site of CABG incision, femoral AT was collected from the area surrounding the saphenous vein (perivascular) and pericardial AT was collected from the site close to the right ventricle. AT was transferred to the lab within 30 minutes and was cultured ex-vivo for 4 hours. MCP-1, IL-6 and adiponectin were quantified in AT culture supernatants.
Results: Hypertension was associated with higher IL-6 in pericardial adipose tissue (14.7±1.8 vs 10.0±1.5 pg/mg p<0.05), MCP-1 (7.07±1.2 vs 2.7±0.6 pg/mg p<0.05) and adiponectin (229.5±15.9 vs 168.9±19.3 pg/mg, p<0.05). Importantly, hypertension was not associated with femoral IL-6 (10.9±1.3 vs 11.9±2.2 pg/mg p=NS), subcutaneous IL-6, (6.9±1.0 vs 8.4±2.1 pg/mg p=NS), femoral MCP-1 (5.7±0.8 vs 4.0±1.1 p=NS), femoral adiponectin (174±34 vs 221±26pg/mg p=NS), subcutaneous MCP-1 (2.37±0.29 vs 3.3±1.5 pg/mg p=NS) or subcutaneous adiponectin (175±18 vs 182±23.2 pg/mg p=NS) AT synthesis. Diastolic (but not systolic) blood pressure was strongly correlated with IL-6 in femoral (rho=0.492, p=0.038) and subcutaneous (rho=0.659, p=0.04) but not pericardial (rho=0.386, p=NS) AT.
Conclusions: Adipokines (IL-6, MCP-1 and adiponectin) synthesized in pericardial adipose tissue are closely associated with the presence of arterial hypertension in patients with advanced atherosclerosis. However, adipokines synthesised in femoral and subcutaneous adipose tissue are strongly associated with the variability of diastolic but not systolic blood pressure.