Abstract 5613: Postconditioning-60 Second by Coronary Angioplasty Significantly Inhibit Myocardial Apoptosis Level in Patients with Acute Myocardial Infarction
Background Postconditioning (Postcon) has been demonstrated to protect the human heart from ischemia/reperfusion injury. The objective of this study was to test the hypothesis that the optimal antiapoptotic could effect relative to different reperfusion-Postcon cycles in patients with acute myocardial infarction (AMI) during primary coronary intervention (PCI).
Methods All 75 patients were randomly assigned to one of three groups before stenting: Routine (n=26): no intervention was given at the onset of reperfusion; Postcon-30s (n=25): 3 cycles of 30s balloon deflation and 30s inflation was repetitively performed; Postcon-60s (n=24): 3 cycles of 60s balloon deflation and 60s inflation was applied. Thirty normal volunteers were enrolled in the present study. ELISA was used to determine the soluble Fas/APO−1 (sFas) and Fas ligand (sFasL) plasma concentration ([sFas]) and ([sFasL]) at baseline and 7d.
Results The [sFas] and [sFasL] in patients with AMI were significantly elevated at baseline as compared with normal volunteers (4.8±0.1* vs.1.7±0.2ng/ml−1 and 3.9±0.2* vs.1.3±0.1ng/ml−1). No further decrease in [sFas] was detected at 7d of reperfusion in Routine (5.1±0.3ng/ml) relative to baseline values. An increase in [sFas] of reperfusion was not altered by Postcon-30s compared with Routine at 7d (4.6±0.3 vs.5.1±0.3ng/ml−1), but was significantly inhibited by Post-60s (3.7±0.1ng/ml−1**) relative to Postcon-30s and Routine. The [sFasL] was significantly increase in the Routine and Postcon-30s groups undergoing reperfusion therapy from 24h to 7d (p<0.05, from 3.0±0.2 to 4.2±0.1ng ml−1 and from 3.3±0.3 to 4.0±0.3ng ml−1), but in Postcon-60s was not significant different (p>0.05, from 3.0±0.2 to 3.1±0.1ng ml−1).
Conclusions The study results suggests that Postcon-60s by coronary angioplasty show significantly inhibit myocardial apoptosis effects and reduce reperfusion injury (*p≪med>0.05 vs. NC; **p≪med>0.05 vs. Postcon-30s and Routine).