Abstract 3900: Stromal Vascular Cell transplantation as an Emerging and Better Regeneration Therapy for Ischemic Heart Failure: Effects and Mechanisms
Mesenchymal stem cells (MSCs) derived from bone marrow (BM) have been used as a potential therapy for chronic heart failure. However, BM aspiration is painful and spinal or general anesthesia is required. The aims of this study were:
to show the feasibility of using adipose-derived stromal vascular fraction (SVF) as an alternative to BM stromal cell for cell transplantation (Tx) into chronic ischemic myocardium; and
to explore underlying mechanisms with focus on anti-inflammation role of engrafted SVF and BM post chronic myocardial infarction (MI) against LV remodeling and cardiac dysfunction.
Four weeks after left coronary artery ligation, rats with MI were randomized into 3 groups. SVF group (n=10) had SVF cell Tx (6x106 cells). BM-MNC group (n=10) received BM mono nuclear cells (6x106) and the control (n=8) had culture medium. Echocardiography performed before cell transplantation, followed by 2 and 4 weeks later. Histological sections were stained with Mason’s Trichrome and immunohistochemical staining for α smooth muscle actin, Factor VIII, CD31, CD3, CD4, CD19, CD20 and CD68. Myocardial mRNA and protein of inflammatory cytokines were measured by RT-PCR and western blot, respectively. Four weeks after transplantation, SVF and BM-MNC groups showed reverse remodeling, in which LV diastolic dimension (LVDd) decreased (SVF, BM-MNC vs Control; 0.91±0.08 to 0.89±0.07 cm, 0.91±0.07 to 0.93±0.09 cm vs 0.91±0.06 to 1.02±0.04), LV systolic dimension (LVDs) decreased (SVF, BM-MNC vs Control; 0.66±0.1 to 0.60±0.13 cm, 0.66±0.1 to 0.66±0.1 cm vs 0.66±0.1 to 0.78±0.05 cm), and fractional shortening increased (SVF, BM Vs Control; 27.4±6.1 to 33.1±9.8%, 28.6±7 to 29.8±5.8% vs 28±7.8 to 23.7±3.7%) compared to control group. Cell transplantation groups decreased protein production and gene expression of inflammatory cytokines TNF-α, IL-6 and inhibited collagen deposition, as well as gene expression of MMP-1 and TIMP-1. Histology revealed higher vascular density in SVF group. Transplantation of adipose derived SVF cells provides a superior benefit to BM-MNC. Anti-inflammation role for SVF and BM transplantation might partly benefit for the cardioprotective effect for chronic ischemic myocardium.