Abstract P31: The Central Nervous System’s Inflammatory Cytokine Response to Global Ischemia Due to Cardiac Arrest
Objective: Pro-inflammatory cytokines have been implicated as culprits in neurotoxicity following ischemia in small animal models of stroke. The aim of this study was to measure the central nervous system (CNS) cytokine response following resuscitation from ventricular fibrillation (VF) in a porcine model of cardiac arrest and global hypoxic ischemia.
Methods: VF was induced electrically in eleven anesthetized swine. Following seven minutes of untreated VF, animals were resuscitated. Cerebrospinal fluid (CSF) and serum was sampled prior to VF and at 60, 120, and 180 minutes post-resuscitation from which levels of TNF-α IL-1β, and IL-6 were measured. Levels were also drawn in three sham pigs, instrumented but not fibrillated.
Results: CSF levels of TNF-α rose following resuscitation (p<0.0001, FIGURE⇓) and were correlated with CSF levels of IL-1β (Spearman rank correlation r=0.68, p<0.0001) and IL-6 (r=0.55, p=0.0003). Changes in cytokine levels were not observed in sham pigs. Only CSF levels of IL-1β were associated with its serum counterpart (p=0.0006).
Conclusions: Measurable increases in pro-inflammatory cytokines in the CSF follow resuscitation from cardiac arrest in this porcine model. The CNS response of all three inflammatory cytokines is highly inter-correlated.