Abstract 6052: Sustained Release of Platelet-Rich Plasma, Cocktail of Potent Angiogenic Cytokines, Restores Blood Perfusion by Recruitment of Bone Marrow Derived Haematopoietic Stem Cells in the Mouse Hind Limb Ischemia
Platelet- rich plasma (PRP) contains several angiogenic cytokines which augment neovascu-larization. However, little is known about the mechanism of PRP related angiogenesis. Recruitment of endothelial progenitor cell to the ischemic site is one of the essential factors for angiogenesis. Sustained release PRP augments neovascularization by recruiting bone marrow derived haematopoietc progenitor cells in mouse hind limb ischemia. Peripheral blood was collected from C57BL/6 mice and PRP was separated by centrifugation of collected blood. Growth factors concentrations were measured in PRP by ELISA. GFP chimeric mice were developed by bone marrow ablation of C57BL/6 mice accompanying with reconstitution by donor tissue from GFP+ transgenic mice. We used gelatin hydrogel microsphere for sustained release carrier of cytokines from PRP. Hind limb ischemia was induced by excising the right femoral artery of GFP chimeric mice. After surgery, mice were randomly assigned to two groups (n=12, each): control (no treatment), 100μl of PRP solution mixed with microspheres (sustained release PRP); applied by single intramuscular injection and observed for 3 days. Tissue blood perfusion was evaluated by laser Doppler perfusion image index (ratio of right/left perfusion; LDPII). Peripheral blood CD34+ cells were detected by fluorescence-activated cell sorting. Local CD34+ cell in ischemic tissue were calculated by double staining with mouse anti GFP antibody and mouse anti - CD34 antibody. The concentration of SDF-1α, PDGF, VEGF, bFGF, IGF-1; blood perfusion, number of local CD34+ cells in ischemic site were significantly higher in group PRP than those in control (table⇓). Sustained release platelet-rich plasma, cocktail of potent angiogenic cytokines, augments neovascularization and blood perfusion possibly due to recruitment as well as homing of haematopoietic stem cell at the ischemic site of mouse hind limb.