Abstract 2: Bone Marrow Mesenchymal Stem Cells Infused Intravenously after Cardiac Resuscitation Improves Cerebral Function
Background: Allogeneic bone marrow mesenchymal stem cells (MSCs) differentiate into neurons. In the present study, we investigated the effects of intravenous infusion of MSCs following successful resuscitation on post-resuscitation neurological outcomes.
Hypothesis: MSCs, when infused intravenously following initial resuscitation, reduce the severity of post-resuscitation cerebral dysfunction.
Methods: Ventricular fibrillation was induced and untreated for 6 min in 20 male Sprague-Dawley rats weighing 450–550g. CPR, including chest compressions and mechanical ventilation, was performed for 6 min prior to attempted defibrillation. All animals were resuscitated. Two hours later, animals were randomized to receive a bolus injection of either 5 × 106 MSCs labeled with PKH26 in phosphate buffer solution (PBS) or PBS as placebo into the right atrium. Survival, adhesive removal, motor test and the Neurological Severity Score [NSS] were measured weekly for a total of 5 weeks. MSCs were counted in 5 μm sections of each harvested brain. Cells from MSCs were identified by immunological markers.
Results: Significant neurological improvement (Table 1⇓) and 5 week survival (9/10 vs. 4/10, p < 0.025) followed injection of MSCs. Labeled MSCs localized to the hippocampus, cortex, pons, medulla, cerebellum were identified. Labeled cells expressed protein markers of neural cells.
Conclusion: MSCs significantly reduce the severity of post-resuscitation cerebral dysfunction and improve survival. Table 1⇓ Adhesive removal, motor test and NSS