Abstract 3741: Human Serum Corin Levels in Health and Atherosclerosis
Introduction: Corin is a type II transmembrane protease responsible for the cleavage of Pro-ANP and Pro- BNP to the active natriuretic peptides, which through their vasodilatory and antiproliferative functions may have a protective effect against atherosclerosis. We wished to determine serum corin level in human and indicate its clinical relevance.
Patients and Methods: An ELISA assay was developed. Serum corin levels were measured in 95 healthy volunteers, age 65±2.85, mean±SEM, 51 males and 44 females, and 173 patients with angiography proven CAD (coronary artery disease). FMD (Flow Mediated Dilatation) using brachial ultrasonography measurements, prior to and following cuff occlusion of the forearm was measured in 111 patients, who had normal baseline FMD, before and 24 hours following routine PCI (FMD patients).
Results: In healthy volunteers, corin level was 1179 ± 58.8 pg/ml, there was a significant difference between males (1490±88.2 pg/ml) and females (798±72.647pg/ml), p<0.0001. Serum corin levels of CAD patients, were significantly lower at 773 ± 21.9 pg/ml, p< 0.0001, with significant difference between 142 males 819±24.0, and 31 females 566.6±30.2 pg/ml, p<0.0001. In 15 of the participates, bloods were redrawn following one week to 1 year after the first sample. There was no difference in the corin levels from the first determination, the difference being only 4.95±0.8%. FMD patients were divided into two groups according to their FMD change post-PCI. Group A: 43 patients showed deterioration of FMD following PCI, 7.8 ± 0.443 %, before, and 5.3 ± 0.335 %, after the procedure, p<0.0001. Group B: 68 patients showed increased FMD following PCI, from 4.4 ± 0.170 % before, to 6.01 ± 0.253 %, after the procedure, p<0.0001. Baseline corin serum level of group A was significantly lower than that of group B, 706 ± 40.225 pg/ml vs. 813 ± 31.25 pg/ml, p =0.0311.
Conclusions: Corin serum levels can accurately and reliably be determined in humans. Patients with CAD have reduced serum corin levels. Reduced serum corin level is associated with increased baseline FMD and loss of endothelial function post PCI.