Abstract 3714: White Wine Mediated Amelioration Of Myocardial Ischemic Reperfusion Injury: Does Colour Matter?
Recent studies on the protection afforded by moderate wine consumption against cardiovascular diseases have focused mainly on the activity of red wine in view of its high content of antioxidant compounds, especially polyphenols. White wine lacks polyphenols because its production differs from that of red wine however it contains hydroxycinnamic acids, such as caffeic acid, and monophenols, such as tyrosol. These compounds possess antioxidant activity and are able to reduce the release of pro-inflammatory cytokines. Therefore, we decided to examine the effect of white wine in myocardial ischemic reperfusion injury. The experimental rats were gavaged with white wine (Soave Suavia “LE RIVE” 2004) at a dosage of 6.5ml/kg/rat/day for 30 days. Rats were divided into 4 groups: Control sham (CS), Wine treated sham (WS), Control + Ischemia(I)/Reperfusion (R) (CIR)and Wine + IR (WIR). All the rats in both IR groups underwent 30 min occlusion of left anterior descending artery followed by 8 & 24 hours and 30 days of reperfusion (R). Significant reduction in infarct size (n=6, 29% vs 39%) and cardiomyocyte apoptosis (n= 6, 274 vs 384 counts/100HPF) were observed in WIR as compared with CIR after 24hrs of reperfusion. Echocardiography demonstrated significant increased fractional shortening (32% vs 22%) and ejection fraction (60% vs 44%) following 3 weeks of reperfusion in WIR rats compared to CIR (n=6). In addition, increased phosphorylation of AKT and eNOS were found in WS (pAKT-1.5 fold, p-eNOS-3.68 fold) and WIR (pAKT-2.7 fold, p-eNOS-3.24 fold) as compared to their respective controls (n=4). The gel-shift analysis demonstrated significant upregulation of DNA binding activity of NF-kappaB in the white wine treated groups. The use of PI-3kinase inhibitor, LY294002 and NF-kappaB inhibitor SN50 completely blocked white wine mediated cardioprotection. This report demonstrated for the first time that the white wine mediated cardioprotection in ischemic reperfused myocardium is through the PI-3kinase/Akt/NF-kappaB survival pathway.