Abstract 2388: Membrane Type 1 Matrix Metalloproteinase Expression On Peripheral Blood Mononuclear Cells After Acute Myocardial Infarction
Background: We reported pericellularly localized membrane-type matrix metalloproteinases (MT-MMPs), which are main activator of secreted latent type MMPs, were highly expressed on circulating peripheral blood mononuclear cells (PBMNCs) from patients with acute myocardial infarction (AMI). However, it has not been clear how changes of MT-MMPs expression after AMI correlate with left ventricular remodeling.
Objective and Methods: To investigate whether peripheral blood levels of MT1-MMP expression on PBMNCs are affected in patients with AMI, serum MMPs and MT1-MMP expression on PBMNCs were measured in 32 patients with AMI, and 15 normal control subjects. PBMNCs were examined for the frequencies of CD14 positive cells expressing MT1-MMP at the single cell level using flow cytometry. Serum levels of MMP-2, -9, TIMP-1, -2 were measured by using ELISA methods.
Results: MT1-MMP expression on PBMNCs in patients with AMI on day 1 was four times higher than that in control subjects and decreased in time-dependent alterations (day 1:61.8 ± 8.6%, day 30:36.5 ± 9.5%, 6-month: 25.7 ± 11.6%, p < 0.05 vs. day 1). Change of MT1-MMP expression from day 1 to day 30 after AMI significantly correlated with change in left ventricular end-diastolic volume from day 30 to 6-month after AMI (r = 0.48, p = 0.024).
Conclusion: MT1-MMP expression varied in a time-dependent manner following AMI and correlated with late change in LVEDV. These finding suggested that the measurement of MT1-MMP on PBMNCs may become a new target of therapy and a predictor of LV remodeling after AMI.