Abstract 2379: No Association of PLIN Polymorphisms with Hemorrhagic and Ischemic Strokes
Background and Purpose Perilipin is a family of proteins that coat the intracellular lipid droplets and expressed in adipocytes, steroid-producing cells and atherosclerotic lesions. I t has been linked to the formation of foam cells and rupture of atherosclerotic plaques. To our knowledge, no other studies have investigated the association between polymorphic variation in PLIN and stroke. We hypothesized that genetic variations in PLIN might contribute to the susceptibility to stroke. We tested the hypothesis in two case-control studies.
Methods Six PLIN tag SNPs (rs7176403, rs8179078, rs6496589, rs8179043, rs894160, rs1052700,) were genotyped in 1571 stroke patients (690 cerebral thrombosis, 429 lacunar infarction, 452 intracerebral hemorrhage) and 1638 controls, by polymerase chain reaction (PCR) followed by ligase detection reaction (LDR) in combination with sequencing, and by PCR and restriction fragment length polymorphism. A SHEsis software was used to analyze pairwise linkage disequilibrium, and haplotype association. The results were confirmed in another independent case-control study, with 120 stroke patients and 240 controls.
Results No association was found between the PLIN variants and stroke in the first study (P>0.05). Six common haplotypes were identified within the study population, none of which was associated with stroke and all its subtypes (P>0.05). The findings were confirmed in the second study (P>0.05).
Conclusions The data represent an important negative finding and that the common variants studied here do not have a major influence on susceptibility to stroke, even if perilipin has been linked to the formation of foam cell and rupture of human atherosclerotic plaques.