Abstract 1547: Multiple Cytokine Measurement in Coronary Artery Disease
Background: Accumulating evidence suggests that inflammation plays an essential role in pathogenesis of atherosclerosis and that circulating inflammatory markers predict cardiovascular event. Although various cytokine levels are measured, their clinical significances have been separately discussed. Different cytokines possess biological overlapping functions, and have the ability to regulate production of other cytokines. Therefore, analysis of the function of the complete set of cytokines expressed at a site of inflammation is more valuable than analysis of a single isolated cytokine. Recent advances in the multianalytic profiling technology using the Luminex micro-beads array system allow simultaneous analysis of multiple cytokines in a single microtiter well.
Methods and Results: Using this system, we measured 10 cytokines, interleukin (IL)-1β, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, tumor necrosis factor (TNF)-α, granulocyte-macrophage colony stimulating factor (GM-CSF) and γ-interferon (IFN-γ), simultaneously in 158 patients with angiographically identified stable coronary artery disease. None of these cytokine levels were correlated with severity of coronary artery disease. During observation of 7 years’ follow-up, onset of cardiovascular events was seen in 59 patients (37%). Although diabetes alone was the predictor of cardiovascular events among risk factors, all of 10 cytokine levels could predict cardiovascular events in univariate logistic regression analysis. However, multiple logistic regression analysis using all of the 10 cytokines and diabetes, namely, predictors in univariate analysis showed that independent predictor was IL-8 (OR; 8.69, 95%CI; 3.54–21.3, P=0.0001) and diabetes (OR; 2.64, 95%CI; 1.13– 6.18, P=0.025).
Conclusion: Activated leukocytes, monocytes, T-lymphocytes and neutrophils, are key players in the inflammatory process of initiation or progression of atherosclerosis, plaque instability or post-PCI restenosis. Since IL-8 is a neutrophil chemokine, our results suggest that neutrophil activation may relate to occurrence of cardiovascular events. Multiple cytokine measurement using the luminex micro-beads array system is a useful tool to evaluate coronary artery disease.