Abstract 1399: Caffeinated Coffee Blunts the Myocardial Protective Effects of Statins Against Ischemia-Reperfusion Injury
Background: Studies have shown controversial results whether caffeinated coffee adversely affects cardiovascular morbidity and mortality. With average daily intake of 3.1 cups of coffee, the daily consumption of caffeine may reach 4 mg/kg, a dose that is sufficient to block the adenosine receptors. As adenosine receptor activation is essential for mediating the infarct-size (IS) limiting effects of statins, we studied whether caffeinated coffee blunts the IS-limiting effects of atorvastatin (ATV).
Methods: Rat received 3-day ATV (10 mg/kg/d) or water by oral gavage once daily. Drinking water were replaced by water + sugar (7.5 g/100 ml), caffeinated coffee with sugar, or decaffeinated coffee with sugar. On the 4th day, rats were anesthetized and underwent 30 min of coronary artery occlusion and 4h reperfusion. Area at risk was assessed by blue dye and IS by triphenyl-tetrazolium-chloride (TTC).
Results: Body weight and area at risk was comparable among groups. In rats not receiving ATV, caffeinated and decaffeinated coffee did not affect IS (Figure⇓). IS was significantly reduced by ATV in the water+sugar and decaffeinated coffee groups. The protective effect of ATV was abrogated in the caffeinated coffee treated rats (Figure⇓).
Conclusions: Coffee did not affect IS in rats not receiving ATV. However, caffeinated coffee, but not decaffeinated coffee, abrogated the IS-limiting effects of ATV by blocking the adenosine receptors.