Abstract 3750: Expression of CD11b Marker in Leukocytes and Nitric Oxide Concentration in Neutrophils and Lymphocytes are Increased in Patients with Acutely Decompensated Heart Failure
Background Non specific inflammation contributes to the pathogenesis of Heart Failure (HF) through a complex interaction between endothelial cells, platelets, and leukocytes. Leukocyte expression of CD11b, a well-known marker of leukocyte activation, and levels of cytoplasmic nitric oxide (NO), an important anti-inflammatory mediator, can be readily measured by flow cytometry.
Objectives: The purpose of this investigation was to determine the degree of expression of activation marker CD11b in leukocytes as well as levels of nitric oxide (NO) in leukocytes in patients with different states of HF using flow cytometry methods.
Methods Patients with known HF were divided in three different groups: NHYA I-II, NYHA III-IV (defined as chronic stable New York Heart Association symptomatology) and Acutely Decompensated HF (defined as HF with a relatively rapid onset of signs and symptoms, resulting in hospitalization or unplanned office or emergency room visits). A peripheral blood sample was obtained in each patient to measure the levels of:
NO in neutrophils;
NO in lymphocytes;
leukocyte expression of activation marker CD11b.
The Kruskal-Wallis stastic was performed to test for differences among groups.
Results: Forty patients with systolic heart failure (age 56 +/−13) with an ejection fraction of 21% (+/−8%) were enrolled. The expression of CD11b in leukocytes was significantly higher in patients with acutely decompensated heart failure than in patients with stable NYHA I-II or III-IV patients. Similarly, intracellular levels of NO in leukocytes was higher in patients with decompensated heart failure. (Table⇓).
Conclusions: HF is associated with an increased leukocyte activation as expressed by cytoplasmic NO levels and expression of CD11b marker, mainly in patients with acutely decompesanted heart failure. Further studies are needed to elucidate the role of leukocyte activation and the role of these markers systolic heart failure.