Abstract 3747: Systemic Oxidative Stress is Increased in Patients With Heart Failure
Oxidative stress may play an important role for the development of heart failure. We tested the hypothesis that expression of thioredoxin (TRX), a stress-inducible and antioxidative protein, was increased in patients with heart failure (HF). 35 patients with HF and 8 controls were studied. The diagnosis of HF was done by cardiac catheterization data with the reference of elevated levels of plasma brain natriuretic peptide (BNP). Skin biopsy, and endomyocardial biopsy in cases of cardiomyopathies, were performed at the time of cardiac catheterization, and the severity of TRX expression was determined immunohistochemically. The serum TRX levels in the patients were higher than in the controls, and correlated positively with the severity of NYHA class. Increased arteriolar TRX expression was found in the skin biopsy in 29 of the patients with HF (82.9%). Also, the severity of endomyocardial TRX expression was increased in HF patients, but did not correlate with BNP levels or any hemodynamic data. Positively TRX expresssed myocytes also showed active DNA damage, detected immunohistochemically by 8-hydroxy-2′-deoxyguanosine. Systemic TRX expression was increased in the patients with HF. The arteriolar and endomyocardial TRX expression may reflect an excessive oxidative stress in HF, where active DNA damage of myocytes may exist.