Abstract 2971: Investigation of Endomyocardial Biopsies from 3345 Patients Over 10 Years - A Retrospective Analysis by Immunohistology and Molecular Biology
Immunohistological and molecular biological investigations of endomyocardial biopsies (EMB) have been demonstrated as important tool for the diagnosis of inflammatory heart muscle diseases with or without viral persistence. From 1993 until 2003 we investigated 3345 EMB from patients with clinically suspected inflammatory heart disease. We used immunohisto-chemical methods (IHC) for the demonstration of infiltrating cells according to the WHF-criteria and polymerase chain reaction (PCR) for the detection of viral or bacterial genomes, which may have pathogenic importance. (Parvovirus PB 19 = PVB 19, Cytomegalovirus = CMV, Adenovirus = ADV, Enterovirus = EV) Results of IHC and PCR investigations: Summary: Inflammatory heart disease was diagnosed by immunohistochemistry in 32.8 % of all investigated patients. Parvovirus B 19 was the most often detected virus in all patient’s subgroups. Detection of PVB 19 genome was significantly correlated with an inflammatory heart disease and reduced ejection fraction (** p < 0.05 group 2 versus 4). In contrast, the high prevalence of PVB 19 in group 3 reflects the patients with dilated cardiomyopathy without inflammation, in whom prevalence of PVB 19 is about 17%. However, the role of PVB 19 in inflammatory heart disease has to be further evaluated.