Abstract 2191: Isolation of Adipose-Derived Stem Cells in Patients with Vascular Disease
Background. Adipose tissue is increasingly being recognized as a source of stem cells for cardiovascular repair. To date, the majority of research has focused on adipose tissue obtained from young, healthy patients undergoing elective plastic surgical procedures. The purpose of this study is to evaluate the isolation of adipose-derived stem cells (ASC) in the patient population most likely to benefit from this technology the elderly patient with vascular disease.
Methods and Results. Adipose tissue was obtained by liposuction of the peri-umbilical abdominal wall from patients undergoing various vascular procedures (n=25, mean 12±5g fat/patient). The adipose tissue was digested with collagenase I and centrifuged to remove mature adipocytes. The number of cells within the resultant stromal-vascular (SV) pellet was quantified (mean 236,000±162,000 cells/gram fat) and plated on polystyrene culture flasks. Following a seven day culture period and negative selection for CD45 and CD31 using magnetic cell sorting (MACS®), the number of ASC was quantified (81,000±62,000cells/gram fat). Flow cytometry revealed the ASC population to be quite homogeneous (>98% positive for CD13, 29 and 90). Correlation between patient demographics (gender, age, BMI, DM, ESRD, PVD) and isolation results revealed a negative correlation for SV cell harvest and DM and ESRD (R=−.41, −.40, P<.05) but a positive correlation with BMI (R=.42, P<.05). ASC isolation correlated positively with BMI (R=.51, P<.05). No correlations with age, gender, or PVD were noted.
Conclusion. These data suggest:
age does not appear to affect the availability of ASC in patients with vascular disease, and
DM and ESRD appears to have a negative effect on the SV pellet but not necessarily the ultimate number of ASC isolated.
Therefore, the use of adipose tissue as a source for adult stem cells in elderly patients with vascular disease appears initially viable.