Abstract 1588: Adipokine Resistin Induces Expression of Adhesion Molecules and Tissue Factor in Human Coronary Artery Endothelial Cells
Objective: Atherosclerosis is characterized by endothelial inflammation and dysfunction. Adipose tissue has been increasingly recognized as an active endocrine organ secreting so-called adipokines, and among them resistin is probably the latest described and the less studied. Resistin has been defined as a novel inflammatory marker in atherosclerosis and its serum levels correlate with coronary artery calcification and multivessel coronary artery disease. The pathophysiology underlying this interplay, however, remains incompletely characterized. The aim of our study is to determine whether resistin might affect the prothrombotic and atherosclerotic characteristics of human coronary artery endothelial cells.
Methods and Results: Incubation of endothelial cells with resistin led to an upregulation of the expression of both ICAM-1 and VCAM-1 (CAMs) as demonstrated by FACS analysis. Moreover, tissue factor (TF) expression and activity were also induced in a dose dependent manner, as shown by FACS analysis and real time PCR, and a specific colorimetric assay respectively. To better investigate the intracellular mechanisms, activation of the transcription factor, NF-kB, was demonstrated by EMSA and by suppression of CAMs and TF expression by the NF-kB inhibitor, pyrrolidine-dithio-carbamate ammonium.
Conclusions: these data confirm that resistin may contribute to atherothrombosis exerting direct effects on human coronary endothelial cells by promoting CAMs and TF expression, supporting the notion that resistin, besides representing a marker of inflammation, is an effector molecule able to induce a pro-atherothrombotic phenotype in cells of the vessel wall.