Abstract 1509: Electrophysiological Characterization of Artificially-Arranged Human Myocardial Cells
Background: Ventricular arrhythmia due to the dysfunction in electrical communication between cardiomyocytes is the common cause of death in patients with CHF. Despite various approaches, the mechanisms behind arrhythmic event are still poorly understood. In this study, for better understanding of the exact changes within/between cardiomyocytes during arrhythmia, initial experiments of electrophysiological characterization were performed in artificially-arranged human myocardial cells.
Methods: Micropatterns were constructed on an adhesive-coated coverslip using the photochemical surface modification technique. We used the recently developed human myocardial cell line (AC-16) which has been demonstrated to exert the characteristics of an adult cardiomyocyte. AC-16 cells grown on the patterned surface were then subjected to electrical stimulation (ES).
Results: When cultured on patterned surface, the AC-16 cells were able to grow into the desired pattern (Fig 1⇓). Immunocytochemical analysis on serially aligned AC-16 cells showed the alterations of cytoskeletal architecture (Fig 2⇓). The gap junction was formed at the boundary of adjacent cells, which was significantly increased with ES (Fig 3⇓), indicating the physiological coupling of the patterned AC-16 cells. Further electrophysiological characterization with optical imaging analysis is now going on.
Conclusion: Electrophysiological characterization of AC-16 cells with the high spatio-resolved micropatterning technique was expected to be a useful tool for investigating cell-cell communication, which might contribute to further understanding of arrhythmogenesis at cellular and tissue levels.