Abstract 1111: Statin Treatment Accelerated Neovessel Formation in the Border Zone of the Infarcted Heart: Architectural Study of Vascular Casts by Scanning Electron Microscopy
Background: Angiogenesis plays an essential role in the healing phase of the infarcted heart. It has been reported that statin treatment enhanced angiogenesis in animal ischemic hind limb model. However, how neovessel formation developed in the infarct border zone and the effect of statin therapy was never examined.
Methods: We introduced myocardial infarction by ligating coronary artery in rats (days 7, −14, and −28 after ligation, n=3 each) and compared with sham-operated rats. The abdominal aorta was cannulated and blood was flushed out from the heart vasculature using cold lactate Ringer solution. The vasculature was slowly perfused with methyl methacrylate and the casting solution was allowed to polymerize. The tissue was then incubated with 10% NaOH. When connective tissues were removed, samples were washed with distilled water. After drying, the casts were mounted and viewed on Scanning Electron Microscope (SEM). We also evaluated microvascular architectural changes associating with statin therapy.
Results: A lot of twisted new developed vessels were observed in the area surrounding the infarct zone after 7 days of ligation in an unorganized manner (figure⇓). Vessels changed thicker at 14 days with more oriented manner and collateral vessels from the patent artery were observed. In day 28, the infarct healing area was fed with organized thick vessels from the non-infarct area. Statin treatment accelerated to sort out the small twisted vessels compared with untreated infarcted heart.
Conclusions: Neovessel architecture in the infarct healing heart was 3-dimensionally observed by SEM. Statin treatment may accelerate neovessel formation in the infarcted heart.