Abstract 1044: Adipogenesis in Obesity is Coupled with Angiogenesis by Circulating Endothelial Progenitor Cells
Ample evidences suggest that angiogenesis plays a pivotal role in adipogenesis in obesity, but little is known about the detailed mechanism. We have developed a visualization technique based on laser confocal microscopy that allows us to analyze angiogenesis and adipogenesis in intact living adipose tissue three-dimensionally in detail. We found that there were close spatial and temporal interrelationships between blood vessel development and adipogenesis, and both were augmented in animal models of obesity (db/db mice, a). Formation of new blood vessels by sprouting from preexisting vasculature was coupled with differentiation of adipocytes (b), and differentiating small adipocytes were surrounded by perivascular cells that presented CD34+, CD133− and CD68+, suggesting that those cells originate from circulating endothelial progenitor cells. Anti-VEGF treatment inhibited not only angiogenesis but also adipogenesis in the db/db mice (a), and local interplay among adipocytes and perivascular cells via humoral factors including VEGF might play essential roles in adipocyte differentiation. In conclusion, our visualization technique provides evidence for the dynamic interactions between adipocyte differentiation and angiogenesis in living adipose tissue in obesity.