Abstract 797: Influence of Regeneration of Human Infarcted Heart Muscle by Intracoronary Autologous Bone Marrow Cell Transplantation in Chronic Coronary Artery Disease on the Mobilization of the BM-CPCs in PB.
Introduction: We recently demonstrated in patients with chronic myocardial infarction by intracoronary transplantation of autologous bone marrow mononuclear cells (BMCs) after 3 months follow-up that the infarct size was reduced by 30%, whereas the global LV ejection fraction increased by 15 % and regional infarct wall movement velocity by 57%. (The IACT Study) We analyzed the influence of regeneration of human infarcted heart muscle by intracoronary cell therapy after 3 months follow up on the mobilization of the bone marrow-derived circulating progenitor cells (BM-CPCs) and NT-proBNP levels in PB
Methods and Results: Peripheral blood concentrations of CD34+ and CD133+ BM-CPCs were measured by flow cytometry in 32 patients with chronic myocardial infarction (5 months to 8 years old) pre, immediately post as well as 3 months after intracoronary cell therapy. NT-proBNP levels were measured in 20 of 32 patients pre and 3 months after intracoronary cell therapy. The concentrations of BM-CPCs significantly increased 3 months after cell therapy compared to pre intracoronary cell therapy (CD34/45+: from 245±97 to 351±177 p=0.003, CD133/45: from 59±27 to 101±57 p<0.001). No significant changes were observed pre intracoronary cell therapy compared to immediately post intracoronary cell therapy (CD34/45+: from 245±97 to 246±97 p=0.9, CD133/45+: 59±27 to 60±27 p=0.85). NT-proBNP levels were significant reduced 3 months after intracoronary cell therapy compared to pre intra coronary cell therapy (n=20, from 136±112 to 66±58 p=0.002)
Conclusion: The regeneration of human infarcted heart muscle by intracoronary autologous BMCs may lead to enhance the mobilization of BM-CPCs in PB and this might be increase the regenerative potency in infracted heart.