Abstract 86: Decline of Circulating Endothelial Progenitor Cells in Acute Stroke Patients
Objective: Recent studies have indicated that endothelial progenitor cells (EPCs) may be an important marker of endothelial function during vascular diseases. The changes of EPCs in course of cerebrovascular diseases are unclear. We studied dynamically EPCs number and other vascular risk factors such as high sensitivity C-reactive protein (hs-CRP) in patients with acute stroke.
Design and Methods: A total of 147 individuals (118 patients with acute stroke including 32 ischemic- and 86 hemorrhage- stroke, 29 healthy controls) were examined. Circulating EPCs were defined by the surface markers CD133/KDR and analyzed by flow cytometry.
Results: Patients with acute stroke had significantly lower numbers of EPCs (0.0383±0.022 /100 PMNCs vs 0.0617±0.0154/100 PMNCs, p<0.05) and higher levels of serum hs-CRP(2.06 mg/dL vs 0.16 mg/dL,P<0.05)than control subjects after adjustment for age, sex, body mass index (BMI) and blood pressure. There were no significant differences in EPCs counts and serum hs-CRP levels between ischemic and hemorrhage stroke. In univariate analyses, BMI (p=0.002), age, SBP, DBP, LDL-, total-cholesterol, and hs-CRP (all p values < 0.001) were inversely correlated with EPC counts. Multivariate analyses revealed SBP and total cholesterol as independent predictors of EPC levels, EPC and hs-CRP as independent predictors of stroke. The clinical course of EPCs change was evaluated in 85 acute stroke patients. The number of EPCs gradually increased on day 7 after acute onset through a decreasing EPCs course and remained significantly higher level during day 7 and 14; and returned to baseline by day 21.
Conclusions: These results suggest a possible contribution of circulating EPCs in acute stroke. SBP and total-cholesterol are independent factors of reduced EPC number. A transient early increment of EPCs might be resulted from the mobilization of EPCs in response to stroke stress.