Abstract 62: Peritoneum as a Novel Organ for Life Support Oxygenation Using Artificial Red Blood Cell (Liposome-Encapsulated Hemoglobin)
Supplemental oxygenation is essentially important in critically ill patients with potentially reversible pulmonary failure such as adult respiratory distress syndrome. Extracorporeal membrane oxygenator (ECMO) and percutaneous cardiopulmonary support (PCPS) have been used for these patients. However, these techniques have so many complications that an additional new therapeutic modality has been expected. To investigate if
a peritoneal cavity can be used as an “extrapulmonary respiration” that is analogous to peritoneal dialysis and
the efficacy of liposome-encapsulated hemoglobin (Artificial Red Blood Cell; Neo Red Cell, NRC), we administered saline, oxygenated human erythrocyte and NRC into peritoneum in experimental rats’ pneumothorax model.
Rats weighing average 250 kg (n=18) were incised in the right chest to make pneumothorax resulted in severe and lethal hypoxia. Rats were allocated into three intra-peritoneum administered groups (each volume of 5 ml) of saline (n=6), erythrocyte (n=6) and NRC (n=6). Survival times from suffering from the pneumothorax were significantly longer in erythrocyte and NRC groups than in saline group (9.2±3.1 min vs. 22.0±4.9 min, 32.0±6.9 min p<0.01). Also, oxygenation levels by arterial blood gas analysis significantly improved in both erythrocyte and NRC groups.
Conclusions: Abdomen (peritoneum) can potentially become “artificial lung” that can be employed in critical care settings. NRC is alternative treatment of washed human erythrocyte.