(Circulation. 1996;94:3103-3108.)
© 1996 American Heart Association, Inc.
Articles |
C-Type Natriuretic Peptide in Human Coronary Atherosclerotic Lesions
the Department of Cardiovascular Pathology, Academic Medical Center, University of Amsterdam, Amsterdam, the Netherlands (T.N., A.C.W., C.M.L., A.E.B.); the Department of Pathology, Osaka City University Medical School, Osaka (M.U.); and the Department of Medicine and Clinical Science, Kyoto University Graduate School of Medicine, Kyoto, Japan (H.I., K.N.).
Correspondence to Prof Anton E. Becker, Department of Cardiovascular Pathology, Academic Medical Center, University of Amsterdam, PO Box 22700, 1100 DE Amsterdam, the Netherlands.
Background C-type natriuretic peptide (CNP) belongs to the natriuretic peptide family and is considered to have regulatory effects on vascular tone and smooth muscle growth. Since these features play a role in atherogenesis, the presence of CNP at such sites was studied.
Methods and Results Thirty-three coronary artery segments were harvested at autopsy: 10 normal, with diffuse intimal thickening, and 23 atherosclerotic lesions. Samples were snap-frozen and processed for immunohistochemical staining. For the identification of CNP, a mouse monoclonal antibody (KY-CNP-1) was used. 1A4, EBM-11 (CD68), and von Willebrand factor antibodies were used to stain smooth muscle cells, macrophages, and endothelial cells, respectively. CNP is present in several cell types. Normal arterial segments show CNP-positive endothelial cells. Hypercellular atherosclerotic lesions show distinct CNP positivity of smooth muscle cells and macrophages but a decrease in positivity of endothelial cells. Advanced atherosclerotic lesions contain CNP-positive macrophages, but the smooth muscle cells within the fibrous cap and the surface endothelial cells are almost all CNP-negative.
Conclusions These observations suggest that CNP has functional significance in atherogenesis.
Key Words: natriuretic peptides • muscle, smooth • atherosclerosis
This article has been cited by other articles:
 |
|
 |
|
  C. M. van der Loos Multiple Immunoenzyme Staining: Methods and Visualizations for the Observation With Spectral Imaging J. Histochem. Cytochem., April 1, 2008; 56(4): 313 - 328. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Park, H. Itoh, K. Yamahara, M. Sone, K. Miyashita, N. Oyamada, N. Sawada, D. Taura, M. Inuzuka, T. Sonoyama, et al. Therapeutic Potential of Atrial Natriuretic Peptide Administration on Peripheral Arterial Diseases Endocrinology, February 1, 2008; 149(2): 483 - 491. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. C. Mendonca, S. Q. Doi, S. Glerum, and D. F. Sellitti Increase of C-Type Natriuretic Peptide Expression by Serum and Platelet-Derived Growth Factor-BB in Human Aortic Smooth Muscle Cells Is Dependent on Protein Kinase C Activation Endocrinology, September 1, 2006; 147(9): 4169 - 4178. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. S. Scotland, M. Cohen, P. Foster, M. Lovell, A. Mathur, A. Ahluwalia, and A. J. Hobbs C-type natriuretic peptide inhibits leukocyte recruitment and platelet-leukocyte interactions via suppression of P-selectin expression PNAS, October 4, 2005; 102(40): 14452 - 14457. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Hobbs, P. Foster, C. Prescott, R. Scotland, and A. Ahluwalia Natriuretic Peptide Receptor-C Regulates Coronary Blood Flow and Prevents Myocardial Ischemia/Reperfusion Injury: Novel Cardioprotective Role for Endothelium-Derived C-Type Natriuretic Peptide Circulation, September 7, 2004; 110(10): 1231 - 1235. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Ohba, N. Hata, and Y. Ohaki Angioscopic Appearance and Histopathology of Coronary Artery Thrombi Asian Cardiovasc Thorac Ann, September 1, 2003; 11(3): 255 - 257. [Abstract] [Full Text]
|
|
|
|
 |
|
 H. Takeuchi, K. Ohmori, I. Kondo, A. Oshita, K. Shinomiya, Y. Yu, Y. Takagi, K. Mizushige, K. Kangawa, and M. Kohno Potentiation of C-type natriuretic peptide with ultrasound and microbubbles to prevent neointimal formation after vascular injury in rats Cardiovasc Res, April 1, 2003; 58(1): 231 - 238. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Yamahara, H. Itoh, T.-H. Chun, Y. Ogawa, J. Yamashita, N. Sawada, Y. Fukunaga, M. Sone, T. Yurugi-Kobayashi, K. Miyashita, et al. Significance and therapeutic potential of the natriuretic peptides/cGMP/cGMP-dependent protein kinase pathway in vascular regeneration PNAS, March 18, 2003; 100(6): 3404 - 3409. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Naruko, M. Ueda, K. Haze, A. C. van der Wal, C. M. van der Loos, A. Itoh, R. Komatsu, Y. Ikura, M. Ogami, Y. Shimada, et al. Neutrophil Infiltration of Culprit Lesions in Acute Coronary Syndromes Circulation, December 3, 2002; 106(23): 2894 - 2900. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Doi, T. Ikeda, H. Itoh, K. Ueyama, K. Hosoda, Y. Ogawa, J. Yamashita, T.-H. Chun, M. Inoue, K. Masatsugu, et al. C-Type Natriuretic Peptide Induces Redifferentiation of Vascular Smooth Muscle Cells With Accelerated Reendothelialization Arterioscler Thromb Vasc Biol, June 1, 2001; 21(6): 930 - 936. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. ITO, R. GOLDSCHMEDING, R. J. BENDE, N. CLAESSEN, M. A. CHAND, L. KLEIJ, T. J. RABELINK, J. J. WEENING, and J. ATEN Kinetics of Connective Tissue Growth Factor Expression during Experimental Proliferative Glomerulonephritis J. Am. Soc. Nephrol., March 1, 2001; 12(3): 472 - 484. [Abstract] [Full Text]
|
|
|
|
 |
|
 K. Morishige, H. Shimokawa, T. Yamawaki, K. Miyata, Y. Eto, T. Kandabashi, K. Yogo, T. Higo, K. Egashira, H. Ueno, et al. Local adenovirus-mediated transfer of C-type natriuretic peptide suppresses vascular remodeling in porcine coronary arteries in vivo J. Am. Coll. Cardiol., March 15, 2000; 35(4): 1040 - 1047. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H.-A. Lehr, C. M. van der Loos, P. Teeling, and A. M. Gown Complete Chromogen Separation and Analysis in Double Immunohistochemical Stains Using Photoshop-based Image Analysis J. Histochem. Cytochem., January 1, 1999; 47(1): 119 - 126. [Abstract] [Full Text]
|
|
|
|
|
|
M. Inoue, H. Itoh, M. Ueda, T. Naruko, A. Kojima, R. Komatsu, K. Doi, Y. Ogawa, N. Tamura, K. Takaya, et al. Vascular Endothelial Growth Factor (VEGF) Expression in Human Coronary Atherosclerotic Lesions : Possible Pathophysiological Significance of VEGF in Progression of Atherosclerosis Circulation, November 17, 1998; 98(20): 2108 - 2116. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.-i. Suga, H. Itoh, Y. Komatsu, H. Ishida, T. Igaki, J. Yamashita, K. Doi, T.-H. Chun, T. Yoshimasa, I. Tanaka, et al. Regulation of Endothelial Production of C-Type Natriuretic Peptide by Interaction between Endothelial Cells and Macrophages Endocrinology, April 1, 1998; 139(4): 1920 - 1926. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Kohno, K. Yokokawa, K. Yasunari, H. Kano, M. Minami, M. Ueda, and J. Yoshikawa Effect of Natriuretic Peptide Family on the Oxidized LDL–Induced Migration of Human Coronary Artery Smooth Muscle Cells Circ. Res., October 19, 1997; 81(4): 585 - 590. [Abstract] [Full Text]
|
|
|
|
 |
|
 T.-H. Chun, H. Itoh, Y. Ogawa, N. Tamura, K. Takaya, T. Igaki, J. Yamashita, K. Doi, M. Inoue, K. Masatsugu, et al. Shear Stress Augments Expression of C-Type Natriuretic Peptide and Adrenomedullin Hypertension, June 1, 1997; 29(6): 1296 - 1302. [Abstract] [Full Text]
|
|
|
|
|
|
N. Ohno, H. Itoh, T. Ikeda, K. Ueyama, K. Yamahara, K. Doi, J. Yamashita, M. Inoue, K. Masatsugu, N. Sawada, et al. Accelerated Reendothelialization With Suppressed Thrombogenic Property and Neointimal Hyperplasia of Rabbit Jugular Vein Grafts by Adenovirus-Mediated Gene Transfer of C-Type Natriuretic Peptide Circulation, April 9, 2002; 105(14): 1623 - 1626. [Abstract] [Full Text] [PDF]
|
|