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(Circulation. 1996;93:1788-1790.)
© 1996 American Heart Association, Inc.

Articles

Restricted Usage of T-Cell Receptor V-Vß Genes in Infiltrating Cells in Aortic Tissue of Patients With Takayasu's Arteritis

Yoshinori Seko, MD; Osamu Sato, MD; Atsuhiko Takagi, MD; Yusuke Tada, MD; Hiroshi Matsuo, MD; Hideo Yagita, PhD; Ko Okumura, PhD; Yoshio Yazaki, MD

From the Third Department of Internal Medicine (Y.S., Y.Y.), Faculty of Medicine, University of Tokyo (Japan); the Department of Immunology (Y.S., H.Y., K.O.), School of Medicine, Juntendo University, Tokyo, Japan; the Institute for Adult Diseases (Y.S.), Asahi Life Foundation, Tokyo, Japan; the Second Department of Surgery (O.S., A.T.), Faculty of Medicine, University of Tokyo (Japan); the Second Department of Surgery (Y.T.), Yamanashi (Japan) Medical College; and the Division of Angiology (H.M.), Department of Internal Medicine, National Cardiovascular Center, Osaka, Japan.

Correspondence to Yoshinori Seko, MD, Third Department of Internal Medicine, Faculty of Medicine, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113, Japan.

Background Infiltration by perforin-secreting killer lymphocytes, such as T cells and natural killer cells, has been shown to be involved in the pathogenesis of vascular cell damage in Takayasu's arteritis.

Methods and Results To investigate the immunological mechanisms involved, especially the nature of T-cell infiltration in Takayasu's arteritis as well as atherosclerosis, we analyzed the expression of T-cell receptor (TCR) V and Vß genes in infiltrating cells in the aortic tissue of patients with Takayasu's arteritis and atherosclerotic aortic aneurysm by polymerase chain reaction (PCR). We also analyzed the expression of cytokine genes by PCR. We found that the repertoires of TCR V as well as Vß gene transcripts in Takayasu's arteritis were restricted. The infiltrating cells expressing V2, V16, V17, Vß7, and Vß13.1 were found in 3 of 4 patients. In contrast, TCR V-Vß repertoires in atherosclerotic aortic aneurysm were polyclonal. There was no significant difference in the pattern of cytokine gene expression between the two diseases.

Conclusions The restricted usage of TCR V as well as Vß genes by infiltrating T cells in Takayasu's arteritis may indicate that a specific antigen in the aortic tissue was targeted. Our findings provide the evidence that distinct immunological mechanisms are involved in the pathogenesis of Takayasu's arteritis and atherosclerotic aortic aneurysm.

Key Words: Takayasu's arteritis • immunology • polymerase chain reaction • atherosclerosis

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