This Article Full Text Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Fukuda, H. Articles by Matsuda, H. Search for Related Content PubMed Articles by Fukuda, H. Articles by Matsuda, H.

(Circulation. 1995;92:413-416.)
© 1995 American Heart Association, Inc.

Articles

Supplement of Nitric Oxide Attenuates Neutrophil-Mediated Reperfusion Injury

Hirotsugu Fukuda, MD; Yoshiki Sawa, MD; Keishi Kadoba, MD; Kazuhiro Taniguchi, MD; Yasuhisa Shimazaki, MD; Hikaru Matsuda, MD

From the First Department of Surgery, Osaka University School of Medicine, Osaka, Japan.

Correspondence to Hikaru Matsuda, MD, First Department of Surgery, Osaka University School of Medicine, 2-2 Yamadaoka, Suita, Osaka, 565 Japan.

Background Nitric oxide (NO) derived from the endothelial cell has been identified as a constitutive chemical mediator that regulates the function of the endothelial cell in association with neutrophil (PMN) adhesion and activation. However, its role in the pathogenesis of myocardial reperfusion injury is not clear.

Methods and Results Fifteen isolated rat hearts were perfused with modified Krebs-Henseleit solution and subjected to 20 minutes of global and normothermic ischemia. Then the hearts were reperfused for 45 minutes with different protocols: the control (C) group was reperfused without PMNs, the P group was reperfused with PMNs, and the N group was reperfused with PMNs and nitroprusside (10^-5 mol/L). The ozone chemiluminescence method was used for direct measurement of NO in the coronary effluent during reperfusion. NO in the coronary effluent in the C group decreased at reperfusion after normoxic perfusion, and this decrease in NO continued for the first 15 minutes of reperfusion. Percentage recovery of left ventricular developed pressure and coronary flow was significantly lower in the P group than that in the N group. Also, the N group had a significantly lesser Luminol-elicited chemiluminescence of the coronary effluent and ratio of PMN adherence to myocardial vasculature compared with the P group.

Conclusions This study demonstrated directly the decrease in NO production during reperfusion and showed that supplement of NO with NO donor attenuated the injury in which PMNs were involved. The results suggest that NO plays a significant role in reperfusion injury and that supplement of NO during reperfusion appears to be useful to attenuate this injury.

Key Words: endothelium-derived factors • neutrophil • reperfusion • molecule

This article has been cited by other articles:

				N. R. Harris Opposing effects of L-NAME on capillary filtration rate in the presence or absence of neutrophils Am J Physiol Gastrointest Liver Physiol, December 1, 1997; 273(6): G1320 - G1325. [Abstract] [Full Text] [PDF]