Circulation, Vol 90, 2886-2890, Copyright © 1994 by American Heart Association
T Endo, T Imaizumi, T Tagawa, M Shiramoto, S Ando and A Takeshita
BACKGROUND: We wished to determine the role of NO in exercise-induced
metabolic forearm vasodilation. METHODS AND RESULTS: Young healthy
volunteers (n = 11) underwent static handgrip exercise (4 to 5 kg, 3
minutes). Forearm blood flow (FBF) measured by strain plethysmography
increased from 4.1 +/- 0.7 mL.min-1.100 mL-1 at rest to 9.8 +/- 1.2
mL.min-1.100 mL-1 immediately after exercise and gradually decreased
thereafter. Exercise was repeated after intrabrachial artery infusion of
NG-monomethyl-L-arginine (L-NMMA) at 4.0 mumol/min for 5 minutes. L- NMMA
did not alter blood pressure and heart rate. L-NMMA decreased FBF at rest
to 2.9 +/- 0.4 mL.min-1.100 mL-1 (P < .01), peak FBF immediately after
exercise to 7.2 +/- 0.7 mL.min-1.100 mL-1 (P < .01), and FBF during the
mid to late phase of metabolic vasodilation (P < .01). Calculated oxygen
consumption during peak exercise was comparable before and after L-NMMA.
Intra-arterially infused L-arginine (10 mg/min, 5 minutes) reversed the
inhibitory effect of L-NMMA. To determine the effect of the decrease in
resting FBF on exercise-induced hyperemia, we normalized FBF after exercise
by resting FBF. The percent increases in FBF after exercise from resting
FBF were similar before and after L-NMMA. Furthermore, we examined the
effect of intra- arterially infused angiotensin II on FBF at rest and after
exercise (n = 7). Angiotensin II decreased FBF at rest from 3.1 +/- 0.3 to
1.8 +/- 0.3 mL.min-1.100 mL-1 (P < .01), peak FBF after exercise from
8.1 +/- 0.5 to 5.6 +/- 0.5 mL.min-1.100 mL-1 (P < .01), and FBF during
the mid to late phase of metabolic vasodilation. The effects of L-NMMA and
angiotensin II on FBF at rest and exercise were similar. CONCLUSIONS: Our
results suggest that L-NMMA decreased FBF after exercise largely by
decreasing resting FBF. These results suggest that NO may not play a
significant role in exercise-induced metabolic arteriolar vasodilation in
the forearm of healthy humans.
ARTICLES
Role of nitric oxide in exercise-induced vasodilation of the forearm
Research Institute of Angiocardiology, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
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