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(Circulation. 2006;113:2120-2127.)
© 2006 American Heart Association, Inc.

Vascular Medicine

CD73/Ecto-5'-Nucleotidase Protects Against Vascular Inflammation and Neointima Formation

Alma Zernecke, MD; Kiril Bidzhekov, PhD; Burcin Özüyaman, MD; Line Fraemohs, MSc; Elisa A. Liehn, MD; Juliane M. Lüscher-Firzlaff, MD; Bernhard Lüscher, PhD; Jürgen Schrader, MD; Christian Weber, MD

From the Institute of Molecular Cardiovascular Research (A.Z., K.B., B.Ö., L.F., E.A.L., C.W.) and the Division of Biochemistry and Molecular Biology (J.M.L.-F., B.L.), Rheinisch-Westfälische Technisch Hochschule (RWTH University), Aachen, Germany, and the Institute of Cardiovascular Physiology (B.Ö., J.S.), Heinrich-Heine University, Düsseldorf, Germany.

Correspondence to Dr Christian Weber, Institut für Kardiovaskuläre Molekularbiologie, Universitätsklinikum Aachen, Pauwelsstraße 30, 52074 Aachen, Germany. E-mail cweber{at}ukaachen.de

Received October 13, 2005; revision received February 28, 2006; accepted March 2, 2006.

Background— Although CD73/ecto-5'-nucleotidase has been implicated in maintaining vasoprotection, its role in regulating endothelial adhesion molecule or inflammatory monocyte recruitment (eg, in the context of vascular injury) remains to be defined.

Methods and Results— Compared with wild-type mice, CD73-deficient (CD73^–/–) mice exhibit increased luminal staining and protein and transcript expression for vascular cell adhesion molecule (VCAM)-1 in carotid arteries. In vitro, aortic endothelial cells (ECs) from CD73^–/– mice display an upregulation of mRNA and protein expression of VCAM-1, associated with increased nuclear factor (NF)-B activity, as determined by chromatin cross-linking and immunoprecipitation or quantitative p65 binding assays. CD73^–/– ECs and carotid arteries perfused ex vivo supported increased monocyte arrest under flow conditions, which was mediated by _4ß1 integrin. After wire injury of carotid arteries, CD73 expression and activity were upregulated in wild-type mice, whereas neointimal plaque formation and macrophage content were increased in CD73^–/– mice versus wild-type mice, concomitant with elevated NF-B activation, luminal VCAM-1 expression, and soluble VCAM-1 concentrations. In contrast, reconstitution of wild-type mice with CD73^–/– versus CD73^+/+ BM did not significantly exacerbate neointima formation. Treatment with the specific A_2A receptor agonist ATL-146e reversed the increased VCAM-1 transcript and protein expression in CD73^–/– ECs and inhibited monocyte arrest on CD73^–/– ECs. Continuous infusion of ATL-146e prevented neointima formation in CD73^–/– mice.

Conclusions— Our data epitomize the importance of vascular CD73 in limiting endothelial activation and monocyte recruitment via generation of adenosine acting through the A_2A receptor, providing a molecular basis for therapeutic protection against vascular inflammation and neointimal hyperplasia.

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