Published online before print January 17, 2005, doi: 10.1161/01.CIR.0000153352.29335.B9
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(Circulation. 2005;111:356-362.)
© 2005 American Heart Association, Inc.
Vascular Medicine |
Adrenomedullin Enhances Angiogenic Potency of Bone Marrow Transplantation in a Rat Model of Hindlimb Ischemia
From the Departments of Regenerative Medicine and Tissue Engineering (T. Iwase, N.N., T. Itoh), Cardiac Physiology (T.F.), and Biochemistry (K.K.), National Cardiovascular Center Research Institute, Osaka, Japan; Departments of Internal Medicine (N.N., M.Y., K.M.), Pathology (H.I.-U.), and Cardiovascular Surgery (S.K.), National Cardiovascular Center, Osaka, Japan; and Department of Medicine and Bioregulatory Sciences (T. Iwase, T.M.), University of Tokushima Graduate School of Medicine, Tokushima, Japan.
Reprint requests to Noritoshi Nagaya, MD, Department of Regenerative Medicine and Tissue Engineering, National Cardiovascular Center Research Institute, 5-7-1 Fujishirodai, Suita, Osaka 565-8565, Japan. E-mail nagayann{at}hsp.ncvc.go.jp
Received June 18, 2004; revision received September 9, 2004; accepted November 3, 2004.
Background— Previous studies have shown that adrenomedullin (AM) inhibits vascular endothelial cell apoptosis and induces angiogenesis. We investigated whether AM enhances bone marrow cell–induced angiogenesis.
Methods and Results— Immediately after hindlimb ischemia was created, rats were randomized to receive AM infusion plus bone marrow–derived mononuclear cell (MNC) transplantation (AM+MNC group), AM infusion alone (AM group), MNC transplantation alone (MNC group), or vehicle infusion (control group). The laser Doppler perfusion index was significantly higher in the AM and MNC groups than in the control group (0.74±0.11 and 0.69±0.07 versus 0.59±0.07, respectively, P<0.01), which suggests the angiogenic potency of AM and MNC. Importantly, improvement in blood perfusion was marked in the AM+MNC group (0.84±0.08). Capillary density was highest in the AM+MNC group, followed by the AM and MNC groups. In vitro, AM inhibited MNC apoptosis, promoted MNC adhesiveness to a human umbilical vein endothelial cell monolayer, and increased the number of MNC-derived endothelial progenitor cells. In vivo, AM administration not only enhanced the differentiation of MNC into endothelial cells but also produced mature vessels that included smooth muscle cells.
Conclusions— A combination of AM infusion and MNC transplantation caused significantly greater improvement in hindlimb ischemia than MNC transplantation alone. This effect may be mediated in part by the angiogenic potency of AM itself and the beneficial effects of AM on the survival, adhesion, and differentiation of transplanted MNCs.
Key Words: peptides • angiogenesis • peripheral vascular disease
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