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(Circulation. 2005;111:1431-1438.)
© 2005 American Heart Association, Inc.

Valvular Heart Disease

Overexpression of Dimethylarginine Dimethylaminohydrolase Reduces Tissue Asymmetric Dimethylarginine Levels and Enhances Angiogenesis

Johannes Jacobi, MD; Karsten Sydow, MD; Georges von Degenfeld, MD; Ying Zhang, PhD; Hayan Dayoub, MD; Bingyin Wang, MD, PhD; Andrew J. Patterson, MD, PhD; Masumi Kimoto, PhD; Helen M. Blau, MD, PhD; John P. Cooke, MD, PhD

From the Division of Cardiovascular Medicine, Stanford University School of Medicine (J.J., K.S., Y.Z., H.D., B.W., J.P.C.), Baxter Laboratory of Genetic Pharmacology, Division of Microbiology and Immunology, Stanford University Medical Center (G.v.D., H.M.B.), and the Division of Anesthesiology, Stanford University Medical Center (A.J.P.), Stanford, Calif; and the Division of Nutritional Science, Okayama Prefectural University, Kuboki, Japan (M.K.).

Correspondence to John P. Cooke, MD, PhD, Division of Cardiovascular Medicine, Stanford University School of Medicine, 300 Pasteur Dr, Stanford, CA 94305. E-mail john.cooke{at}stanford.edu

Received August 5, 2004; revision received October 28, 2004; accepted November 24, 2004.

Background— This study was designed to determine whether overexpression of the enzyme dimethylarginine dimethylaminohydrolase (DDAH) could enhance angiogenesis by reducing levels of the endogenous nitric oxide synthase (NOS) inhibitor asymmetric dimethylarginine (ADMA).

Methods and Results— In DDAH1 transgenic (TG) and wild-type mice (each n=42), the role of DDAH overexpression on angiogenesis was studied by use of the disk angiogenesis system and a murine model of hindlimb ischemia (each n=21). After surgery, animals were treated with either PBS or the NOS inhibitors ADMA or N-nitro-L-arginine methyl ester (L-NAME; each 250 µmol · kg^–1 · d^–1) by use of osmotic minipumps (each n=7). L-NAME was chosen to study an inhibitor that is not degraded by DDAH. Neovascularization in the disk angiogenesis system was impaired by both NOS inhibitors; however, TG animals were resistant to the effects of ADMA on neovascularization. Similarly, TG mice were more resistant to the inhibitory effect of ADMA on angioadaptation (angiogenesis and arteriogenesis) after hindlimb ischemia, as assessed by fluorescent microsphere studies and postmortem microangiograms. Enhanced neovascularization and limb perfusion in TG mice were associated with reduced plasma and tissue ADMA levels and enhanced tissue NOS enzyme activity.

Conclusions— We describe a novel mechanism by which DDAH regulates postnatal neovascularization. Therapeutic manipulation of DDAH expression or activity may represent a novel approach to restore tissue perfusion.

Key Words: nitric oxide • arginine • aminohydrolases • angiogenesis • ischemia

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