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(Circulation. 2004;110:2673-2677.)
© 2004 American Heart Association, Inc.

Molecular Cardiology

Integrin Profile and In Vivo Homing of Human Smooth Muscle Progenitor Cells

Arjun Deb, MD^*; Kimberly A. Skelding, MD^*; Shaohua Wang, MD; Margo Reeder, BS; David Simper, MD; Noel M. Caplice, MD, PhD

From the Division of Cardiovascular Diseases and Molecular Medicine Program, Mayo Clinic, Rochester, Minn.

Correspondence to Noel M. Caplice MD, PhD, Mayo Clinic, GU 1801, 200 First St SW, Rochester, MN 55905. E-mail caplice.noel{at}mayo.edu

Received October 20, 2003; de novo received June 9, 2004; revision received June 23, 2004; accepted June 23, 2004.

Background— Recently, we identified circulating smooth muscle progenitor cells (SPCs) in human peripheral blood. The integrin profile of such progenitors is currently unknown and may affect their in vivo homing characteristics. In this study, we determined the integrin profile of vascular progenitors and SPC adhesion to extracellular matrix (ECM) proteins in vitro and in vivo.

Methods and Results— SPCs and endothelial progenitor cells (EPCs) were isolated from peripheral blood of healthy human subjects, and expression of surface integrins and adhesion to several vascular ECM proteins were determined. Homing of SPCs in vivo to specific ECM protein was determined by intracoronary infusion of fluorescent SPCs into porcine coronary arteries containing a fibronectin-coated mesh stent. SPCs had high expression of ß₁ integrin, moderate expression of ₁, low levels of _vß₃, and did not express _vß₅, ß₂, ₂ß₁, or ₄ß₁ integrins. In contrast, EPCs had high expression of ₂ß₁, _vß₃, _vß₅, ß₁, and ₁ and minimal expression of ₄ß₁. Moreover, SPCs showed increased adherence to fibronectin and collagen type I compared with vitronectin, consistent with their integrin profile, and demonstrated a similar degree of in vivo attachment to fibronectin-coated mesh.

Conclusions— These data for the first time show a spectrum of integrin expression on vascular progenitors and suggest the potential importance of integrins in mediating adherence of SPCs to specific ECM both in vitro and in vivo.

Key Words: integrins • muscle, smooth • stem cells • extracellular matrix

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