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(Circulation. 2003;108:610.)
© 2003 American Heart Association, Inc.

Basic Science Reports

Therapeutic Induction of Arteriogenesis in Hypoperfused Rat Brain Via Granulocyte-Macrophage Colony-Stimulating Factor

Ivo R. Buschmann, MD^*; Hans-Jörg Busch, MD^*; Günter Mies, MD; Konstantin-Alexander Hossmann, MD

From the Albert Ludwigs University Freiburg (I.R.B., H.-J.B.), Research Group for Experimental and Clinical Arteriogenesis, Department of Cardiology and Angiology, Freiburg im Breisgau, Germany, and Max-Planck-Institute for Neurological Research (H.-J.B., G.M., K.-A.H.), Department for Experimental Neurology, Cologne, Germany.

Correspondence to Ivo Buschmann, MD, Research Group for Experimental and Clinical Arteriogenesis, Department for Cardiology and Angiology, Albert Ludwigs University, Breisacher Strasse 66, Freiburg im Breisgau, Germany. E-mail buschmann{at}med1.ukl.uni-freiburg.de

Received February 11, 2003; revision received April 1, 2003; accepted April 4, 2003.

Background— Colony-stimulating factors (CSFs) have been shown to effectively induce arteriogenesis in the hindlimb. Moreover, clinical trials demonstrated positive effects of CSFs on arteriogenesis in patients with coronary artery disease. However, patients with cerebrovascular disease have not yet profited from treatments aimed at the growth of brain vessels. Thus far, angiogenesis studies have failed to demonstrate improvement of stroke outcome. Arteriogenesis differs from angiogenesis in that it substitutes arterial collaterals for the occluded artery.

Methods and Results— We tested in a novel brain arteriogenesis rat model (occlusion of vertebral plus left carotid artery [3-VO]) the application of CSFs or saline over 7 or 21 days. On 3-VO postmortem, latex perfusion demonstrated a time- and treatment-dependent arteriogenesis of the posterior cerebral artery (PCA). In saline-treated animals, the PCA diameter increased by 39%; in granulocyte-macrophage (GM)-CSF-treated animals, this increase was significantly faster (72% after 1 week). Functionally, saline-treated animals exhibited a decline of CO₂ reactivity (mm Hg) from 1.48% to 0.1% compared with GM-CSF–treated animals (1.43% arterial pCO₂ change after 1 week). This difference remained significant after 3 weeks. This functional improvement correlated with increased numbers of CD68-positive macrophages in histological sections of the PCA in GM-CSF–treated animals and only a few macrophages in saline-treated animals.

Conclusions— To the best of our knowledge, this is the first report of stimulation of arteriogenesis in the brain. The subcutaneous application of GM-CSF led to functional improvement of brain hemodynamic parameters.

Key Words: arteries • stroke • cerebrovascular circulation • drugs

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