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(Circulation. 2003;108:II-264.)
© 2003 American Heart Association, Inc.

Cell Transplantation and Tissue Engineering

Comparison of Intracardiac Cell Transplantation: Autologous Skeletal Myoblasts Versus Bone Marrow Cells

Richard B. Thompson, MD; Sitaram M. Emani, MD; Bryce H. Davis, BSE; Ewout J. van den Bos, MD^*; Yoshihisa Morimoto, MD, PhD; Damian Craig, MSE; Donald Glower, MD; Doris A. Taylor, PhD

From the Departments of Surgery and Medicine, Duke University Medical Center and Department of Biomedical Engineering, Duke University, Durham, NC

Correspondence to Doris A Taylor, Ph.D., Box 3345, Duke University Medical Center, Durham, NC 27710. Phone: 919-684-5398; Fax: 919-684-8907

Background— Multiple cell types are being proposed for cardiac repair, but side-by-side comparisons are lacking. We tested the hypothesis that intracardiac transplantation of autologous bone marrow- or skeletal muscle-derived progenitor cells improve regional heart function to a similar degree.

Methods and Results— Thirty-nine New Zealand White rabbits underwent cryoinjury of the left ventricle and simultaneous hind limb bone marrow aspiration or soleus muscle biopsy. Both muscle and bone marrow cells were expanded in vitro. After 2 weeks, 10⁸ skeletal muscle (SM group) or bone marrow-derived progenitor cells (BM group) were injected into the cryoinjured region (SM: n=12; BM: n=8). Medium alone was injected into the remaining animals (Control: n=16). Regional systolic function was measured using micromanometry and sonomicrometry at baseline, before, and 4 weeks after cell injection. Cell treatment resulted in a similar degree of improvement in a derivative of stroke work in the SM and BM groups (P=0.0026 and P=0.0085 versus Control, respectively). No significant difference was seen between BM and SM groups (P=0.9). On histology, engrafted cells were found in all of the cell treated animals. Injected myoblasts formed myotubes or muscle cells throughout the scar that expressed slow and fast myosin heavy chain. A subset of bone marrow cells differentiated toward a myogenic phenotype, as indicated by expression of desmin and -sarcomeric actin in the engrafted areas.

Conclusion— Transplantation and myogenic differentiation of bone marrow-derived progenitor cells increased regional systolic heart function after myocardial injury to a similar degree as skeletal myoblasts.

Key Words: cellular cardiomyoplasty • myoblasts • cell transplantation • stem cells • infarction