Circulation. 2003;107:1024-1032
Published online before print February 3, 2003, doi: 10.1161/01.CIR.0000051460.85800.BB
Published online before print February 3, 2003, doi: 10.1161/01.CIR.0000051460.85800.BB
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
(Circulation. 2003;107:1024.)
© 2003 American Heart Association, Inc.
Basic Science Reports |
Transdifferentiation of Blood-Derived Human Adult Endothelial Progenitor Cells Into Functionally Active Cardiomyocytes
From Molecular Cardiology, Department of Internal Medicine IV (C.B., S.R., C.U., A.A., A.M.Z., S.D.), and the Institute for Cardiovascular Physiology (R.P.B., R.P., I.F., R.B), University of Frankfurt, Frankfurt, Germany.
Correspondence to Stefanie Dimmeler, Department of Molecular Cardiology, University of Frankfurt, Theodor Stern-Kai 7, 60590 Frankfurt, Germany. E-mail dimmeler{at}em.uni-frankfurt.de
Background— Further to promoting angiogenesis, cell therapy may be an approach for cardiac regeneration. Recent studies suggest that progenitor cells can transdifferentiate into other lineages. However, the transdifferentiation potential of endothelial progenitor cells (EPCs) is unknown.
Methods and Results— EPCs were obtained from peripheral blood mononuclear cells of healthy adults or coronary artery disease (CAD) patients by cultivating with endothelial cell medium and growth factors. After 3 days, >95% of adherent cells were functionally and phenotypically EPCs. Diacetylated LDL–labeled EPCs were then cocultivated with rat cardiomyocytes for 6 days, resulting in significant increases of EPC cell length and size to a cardiomyocyte-like morphology. Biochemically, 9.94±1.39% and 5.04±1.09% of EPCs from healthy adults (n=15) or CAD patients (n=14, P<0.01 versus healthy adults), respectively, expressed 
-sarcomeric actinin as measured by flow cytometry. Immunocytochemistry showed that human EPCs expressed -sarcomeric actinin, cardiac troponin I (both with partial sarcomeric organization), atrial natriuretic peptide, and myocyte enhancer factor 2. Fluo 4 imaging demonstrated calcium transients synchronized with adjacent rat cardiomyocytes in transdifferentiated human EPCs. Single-cell microinjection of Lucifer yellow and calcein-AM labeling of cardiomyocytes demonstrated gap junctional communication between 51±7% of EPCs (16 hours after labeling, n=4) and cardiomyocytes. EPC transdifferentiation into cardiomyocytes was not observed with conditioned medium but in coculture with paraformaldehyde-fixed cardiomyocytes.
Conclusions— EPCs from healthy volunteers and CAD patients can transdifferentiate in vitro into functionally active cardiomyocytes when cocultivated with rat cardiomyocytes. Cell-to-cell contact but not cellular fusion mediates EPC transdifferentiation. The therapeutic use of autologous EPCs may aid cardiomyocyte regeneration in patients with ischemic heart disease.
Key Words: myocytes • physiology • cells • endothelium
This article has been cited by other articles:
 |
|
 |
|
  A. Barbuti The 'hearty' fat: adipocytes as a source of functional cardiomyocytes Cardiovasc Res, November 23, 2009; (2009) cvp358v2. [Full Text] [PDF]
|
|
|
|
 |
|
 J. L. Herrmann, A. M. Abarbanell, B. R. Weil, Y. Wang, M. Wang, J. Tan, and D. R. Meldrum Cell-based therapy for ischemic heart disease: a clinical update. Ann. Thorac. Surg., November 1, 2009; 88(5): 1714 - 1722. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Tommila, A. Jokilammi, P. Terho, T. Wilson, R. Penttinen, and E. Ekholm Hydroxyapatite coating of cellulose sponges attracts bone-marrow-derived stem cells in rat subcutaneous tissue J R Soc Interface, October 6, 2009; 6(39): 873 - 880. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. V Arom, P. Ruengsakulrach, M. Belkin, and M. Tiensuwan Intramyocardial Angiogenic Cell Precursors in Nonischemic Dilated Cardiomyopathy Asian Cardiovasc Thorac Ann, August 1, 2009; 17(4): 382 - 388. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. D. Boudoulas and A. K. Hatzopoulos Cardiac repair and regeneration: the Rubik's cube of cell therapy for heart disease Dis. Model. Mech., July 1, 2009; 2(7-8): 344 - 358. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Mollmann, H. Nef, A. Elsasser, and C. Hamm Stem cells in myocardial infarction: from bench to bedside Heart, March 15, 2009; 95(6): 508 - 514. [Full Text] [PDF]
|
|
|
|
 |
|
 K. Yamahara and H. Itoh Potential use of endothelial progenitor cells for regeneration of the vasculature Therapeutic Advances in Cardiovascular Disease, February 1, 2009; 3(1): 17 - 27. [Abstract] [PDF]
|
|
|
|
 |
|
 G. Suzuki, V. Iyer, T. Cimato, and J. M. Canty Jr Pravastatin Improves Function in Hibernating Myocardium by Mobilizing CD133+ and cKit+ Bone Marrow Progenitor Cells and Promoting Myocytes to Reenter the Growth Phase of the Cardiac Cell Cycle Circ. Res., January 30, 2009; 104(2): 255 - 264. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A Ghodsizad, M Niehaus, G Kogler, U Martin, P Wernet, C Bara, N Khaladj, A Loos, M Makoui, J Thiele, et al. Transplanted human cord blood-derived unrestricted somatic stem cells improve left-ventricular function and prevent left-ventricular dilation and scar formation after acute myocardial infarction Heart, January 1, 2009; 95(1): 27 - 35. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Ziebart, C.-H. Yoon, T. Trepels, A. Wietelmann, T. Braun, F. Kiessling, S. Stein, M. Grez, C. Ihling, M. Muhly-Reinholz, et al. Sustained Persistence of Transplanted Proangiogenic Cells Contributes to Neovascularization and Cardiac Function After Ischemia Circ. Res., November 21, 2008; 103(11): 1327 - 1334. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Reinecke, E. Minami, W.-Z. Zhu, and M. A. Laflamme Cardiogenic Differentiation and Transdifferentiation of Progenitor Cells Circ. Res., November 7, 2008; 103(10): 1058 - 1071. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Murasawa and T. Asahara Review: Cardiogenic potential of endothelial progenitor cells Therapeutic Advances in Cardiovascular Disease, October 1, 2008; 2(5): 341 - 348. [Abstract] [PDF]
|
|
|
|
 |
|
 N. Derval, L. Barandon, P. Dufourcq, L. Leroux, J.-M. D. Lamaziere, D. Daret, T. Couffinhal, and C. Duplaa Epicardial deposition of endothelial progenitor and mesenchymal stem cells in a coated muscle patch after myocardial infarction in a murine model. Eur. J. Cardiothorac. Surg., August 1, 2008; 34(2): 248 - 254. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Rupp, M. Koyanagi, M. Iwasaki, J. Bauer, S. von Gerlach, D. Schranz, A. M. Zeiher, and S. Dimmeler Characterization of long-term endogenous cardiac repair in children after heart transplantation Eur. Heart J., August 1, 2008; 29(15): 1867 - 1872. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Rupp, M. Koyanagi, M. Iwasaki, F. Diehl, P. Bushoven, D. Schranz, A. M. Zeiher, and S. Dimmeler Genetic proof-of-concept for cardiac gene expression in human circulating blood-derived progenitor cells. J. Am. Coll. Cardiol., June 10, 2008; 51(23): 2289 - 2290. [Full Text] [PDF]
|
|
|
|
|
|
H Ince, M Valgimigli, M Petzsch, J S. de Lezo, F Kuethe, S Dunkelmann, G Biondi-Zoccai, and C A Nienaber Cardiovascular events and re-stenosis following administration of G-CSF in acute myocardial infarction: systematic review and meta-analysis Heart, May 1, 2008; 94(5): 610 - 616. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Foteinos, Y. Hu, Q. Xiao, B. Metzler, and Q. Xu Rapid Endothelial Turnover in Atherosclerosis-Prone Areas Coincides With Stem Cell Repair in Apolipoprotein E-Deficient Mice Circulation, April 8, 2008; 117(14): 1856 - 1863. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. V Arom, P. Ruengsakulrach, and V. Jotisakulratana Intramyocardial Angiogenic Cell Precursor Injection for Cardiomyopathy Asian Cardiovasc Thorac Ann, April 1, 2008; 16(2): 143 - 148. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Orlandi, F. Pagani, D. Avitabile, G. Bonanno, G. Scambia, E. Vigna, F. Grassi, F. Eusebi, S. Fucile, M. Pesce, et al. Functional properties of cells obtained from human cord blood CD34+ stem cells and mouse cardiac myocytes in coculture Am J Physiol Heart Circ Physiol, April 1, 2008; 294(4): H1541 - H1549. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. S. Fazel, L. Chen, D. Angoulvant, S.-H. Li, R. D. Weisel, A. Keating, and R.-K. Li Activation of c-kit is necessary for mobilization of reparative bone marrow progenitor cells in response to cardiac injury FASEB J, March 1, 2008; 22(3): 930 - 940. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Burghoff, Z. Ding, S. Godecke, A. Assmann, A. Wirrwar, D. Buchholz, O. Sergeeva, C. Leurs, H. Hanenberg, H.-W. Muller, et al. Horizontal gene transfer from human endothelial cells to rat cardiomyocytes after intracoronary transplantation Cardiovasc Res, February 1, 2008; 77(3): 534 - 543. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Koyanagi, P. Bushoven, M. Iwasaki, C. Urbich, A. M. Zeiher, and S. Dimmeler Notch Signaling Contributes to the Expression of Cardiac Markers in Human Circulating Progenitor Cells Circ. Res., November 26, 2007; 101(11): 1139 - 1145. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Schroeder, R. Popp, B. Wiegand, J. Altschmied, and J. Haendeler Nuclear Redox-Signaling Is Essential for Apoptosis Inhibition in Endothelial Cells Important Role for Nuclear Thioredoxin-1 Arterioscler Thromb Vasc Biol, November 1, 2007; 27(11): 2325 - 2331. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. F.M. Segers, T. Tokunou, L. J. Higgins, C. MacGillivray, J. Gannon, and R. T. Lee Local Delivery of Protease-Resistant Stromal Cell Derived Factor-1 for Stem Cell Recruitment After Myocardial Infarction Circulation, October 9, 2007; 116(15): 1683 - 1692. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Bearzi, M. Rota, T. Hosoda, J. Tillmanns, A. Nascimbene, A. De Angelis, S. Yasuzawa-Amano, I. Trofimova, R. W. Siggins, N. LeCapitaine, et al. Human cardiac stem cells PNAS, August 28, 2007; 104(35): 14068 - 14073. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Grote, G. Salguero, M. Ballmaier, M. Dangers, H. Drexler, and B. Schieffer The angiogenic factor CCN1 promotes adhesion and migration of circulating CD34+ progenitor cells: potential role in angiogenesis and endothelial regeneration Blood, August 1, 2007; 110(3): 877 - 885. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. M. Vartanian and R. Sarkar Therapeutic Angiogenesis Vascular and Endovascular Surgery, July 1, 2007; 41(3): 173 - 185. [Abstract] [PDF]
|
|
|
|
|
|
K. Guan, S. Wagner, B. Unsold, L. S. Maier, D. Kaiser, B. Hemmerlein, K. Nayernia, W. Engel, and G. Hasenfuss Generation of Functional Cardiomyocytes From Adult Mouse Spermatogonial Stem Cells Circ. Res., June 8, 2007; 100(11): 1615 - 1625. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. L.T. Ballard and J. M. Edelberg Stem Cells and the Regeneration of the Aging Cardiovascular System Circ. Res., April 27, 2007; 100(8): 1116 - 1127. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Caballero, N. Sengupta, A. Afzal, K.-H. Chang, S. Li Calzi, D. L. Guberski, T. S. Kern, and M. B. Grant Ischemic Vascular Damage Can Be Repaired by Healthy, but Not Diabetic, Endothelial Progenitor Cells Diabetes, April 1, 2007; 56(4): 960 - 967. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Brade, J. Manner, and M. Kuhl The role of Wnt signalling in cardiac development and tissue remodelling in the mature heart Cardiovasc Res, November 1, 2006; 72(2): 198 - 209. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K.-L. Ang, L. Takura Shenje, L. Srinivasan, and M. Galinanes Repair of the damaged heart by bone marrow cells: from experimental evidence to clinical hope. Ann. Thorac. Surg., October 1, 2006; 82(4): 1549 - 1558. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Matsumoto, A. Kawamoto, R. Kuroda, M. Ishikawa, Y. Mifune, H. Iwasaki, M. Miwa, M. Horii, S. Hayashi, A. Oyamada, et al. Therapeutic Potential of Vasculogenesis and Osteogenesis Promoted by Peripheral Blood CD34-Positive Cells for Functional Bone Healing Am. J. Pathol., October 1, 2006; 169(4): 1440 - 1457. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. M. Davidson and D. M. Yellon Dissecting out the mechanism of cardioprotection by endogenous erthyropoietin using genetic engineering Cardiovasc Res, August 1, 2006; 71(3): 408 - 410. [Full Text] [PDF]
|
|
|
|
|
|
W.-H. Zimmermann, M. Didie, S. Doker, I. Melnychenko, H. Naito, C. Rogge, M. Tiburcy, and T. Eschenhagen Heart muscle engineering: An update on cardiac muscle replacement therapy Cardiovasc Res, August 1, 2006; 71(3): 419 - 429. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Misao, G. Takemura, M. Arai, T. Ohno, H. Onogi, T. Takahashi, S. Minatoguchi, T. Fujiwara, and H. Fujiwara Importance of recruitment of bone marrow-derived CXCR4+ cells in post-infarct cardiac repair mediated by G-CSF Cardiovasc Res, August 1, 2006; 71(3): 455 - 465. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. H. Lee and J. H. P. Hui The potential of stem cells in orthopaedic surgery J Bone Joint Surg Br, July 1, 2006; 88-B(7): 841 - 851. [Full Text] [PDF]
|
|
|
|
|
|
N. Ma, Y. Ladilov, J. M. Moebius, L. Ong, C. Piechaczek, A. David, A. Kaminski, Y.-H. Choi, W. Li, D. Egger, et al. Intramyocardial delivery of human CD133+ cells in a SCID mouse cryoinjury model: Bone marrow vs. cord blood-derived cells Cardiovasc Res, July 1, 2006; 71(1): 158 - 169. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. L. Semenza Therapeutic Angiogenesis: Another Passing Phase? Circ. Res., May 12, 2006; 98(9): 1115 - 1116. [Full Text] [PDF]
|
|
|
|
|
|
A. Furuta, S. Miyoshi, Y. Itabashi, T. Shimizu, S. Kira, K. Hayakawa, N. Nishiyama, K. Tanimoto, Y. Hagiwara, T. Satoh, et al. Pulsatile Cardiac Tissue Grafts Using a Novel Three-Dimensional Cell Sheet Manipulation Technique Functionally Integrates With the Host Heart, In Vivo Circ. Res., March 17, 2006; 98(5): 705 - 712. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. G.P. Welt and D. W. Losordo Cell Therapy for Acute Myocardial Infarction: Curb Your Enthusiasm? Circulation, March 14, 2006; 113(10): 1272 - 1274. [Full Text] [PDF]
|
|
|
|
|
|
H. Iwasaki, A. Kawamoto, M. Ishikawa, A. Oyamada, S. Nakamori, H. Nishimura, K. Sadamoto, M. Horii, T. Matsumoto, S. Murasawa, et al. Dose-Dependent Contribution of CD34-Positive Cell Transplantation to Concurrent Vasculogenesis and Cardiomyogenesis for Functional Regenerative Recovery After Myocardial Infarction Circulation, March 14, 2006; 113(10): 1311 - 1325. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Gruh, J. Beilner, U. Blomer, A. Schmiedl, I. Schmidt-Richter, M.-L. Kruse, A. Haverich, and U. Martin No Evidence of Transdifferentiation of Human Endothelial Progenitor Cells Into Cardiomyocytes After Coculture With Neonatal Rat Cardiomyocytes Circulation, March 14, 2006; 113(10): 1326 - 1334. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Anghelina, P. Krishnan, L. Moldovan, and N. I. Moldovan Monocytes/Macrophages Cooperate with Progenitor Cells during Neovascularization and Tissue Repair: Conversion of Cell Columns into Fibrovascular Bundles Am. J. Pathol., February 1, 2006; 168(2): 529 - 541. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Eschenhagen and W. H. Zimmermann Engineering Myocardial Tissue Circ. Res., December 9, 2005; 97(12): 1220 - 1231. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Ince, M. Petzsch, H. D. Kleine, H. Schmidt, T. Rehders, T. Korber, C. Schumichen, M. Freund, and C. A. Nienaber Preservation From Left Ventricular Remodeling by Front-Integrated Revascularization and Stem Cell Liberation in Evolving Acute Myocardial Infarction by Use of Granulocyte-Colony-Stimulating Factor (FIRSTLINE-AMI) Circulation, November 15, 2005; 112(20): 3097 - 3106. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Erbs, A. Linke, V. Adams, K. Lenk, H. Thiele, K.-W. Diederich, F. Emmrich, R. Kluge, K. Kendziorra, O. Sabri, et al. Transplantation of Blood-Derived Progenitor Cells After Recanalization of Chronic Coronary Artery Occlusion: First Randomized and Placebo-Controlled Study Circ. Res., October 14, 2005; 97(8): 756 - 762. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. T. Chan, S. H. Li, and S. Verma Nocturnal hemodialysis is associated with restoration of impaired endothelial progenitor cell biology in end-stage renal disease Am J Physiol Renal Physiol, October 1, 2005; 289(4): F679 - F684. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Siepe, C. Heilmann, P. von Samson, P. Menasche, and F. Beyersdorf Stem cell research and cell transplantation for myocardial regeneration Eur. J. Cardiothorac. Surg., August 1, 2005; 28(2): 318 - 324. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Schulze, F. Belema-Bedada, A. Technau, and T. Braun Mesenchymal stem cells are recruited to striated muscle by NFAT/IL-4-mediated cell fusion Genes & Dev., August 1, 2005; 19(15): 1787 - 1798. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Pfister, F. Mouquet, M. Jain, R. Summer, M. Helmes, A. Fine, W. S. Colucci, and R. Liao CD31- but Not CD31+ Cardiac Side Population Cells Exhibit Functional Cardiomyogenic Differentiation Circ. Res., July 8, 2005; 97(1): 52 - 61. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Murasawa, A. Kawamoto, M. Horii, S. Nakamori, and T. Asahara Niche-Dependent Translineage Commitment of Endothelial Progenitor Cells, Not Cell Fusion in General, Into Myocardial Lineage Cells Arterioscler Thromb Vasc Biol, July 1, 2005; 25(7): 1388 - 1394. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. M. Leone, S. Rutella, G. Bonanno, A. Abbate, A. G. Rebuzzi, S. Giovannini, M. Lombardi, L. Galiuto, G. Liuzzo, F. Andreotti, et al. Mobilization of bone marrow-derived stem cells after myocardial infarction and left ventricular function Eur. Heart J., June 2, 2005; 26(12): 1196 - 1204. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Lagostena, D. Avitabile, E. De Falco, A. Orlandi, F. Grassi, M. G. Iachininoto, G. Ragone, S. Fucile, G. Pompilio, F. Eusebi, et al. Electrophysiological properties of mouse bone marrow c-kit+ cells co-cultured onto neonatal cardiac myocytes Cardiovasc Res, June 1, 2005; 66(3): 482 - 492. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Koyanagi, R. P. Brandes, J. Haendeler, A. M. Zeiher, and S. Dimmeler Cell-to-Cell Connection of Endothelial Progenitor Cells With Cardiac Myocytes by Nanotubes: A Novel Mechanism for Cell Fate Changes? Circ. Res., May 27, 2005; 96(10): 1039 - 1041. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Koyanagi, J. Haendeler, C. Badorff, R. P. Brandes, J. Hoffmann, P. Pandur, A. M. Zeiher, M. Kuhl, and S. Dimmeler Non-canonical Wnt Signaling Enhances Differentiation of Human Circulating Progenitor Cells to Cardiomyogenic Cells J. Biol. Chem., April 29, 2005; 280(17): 16838 - 16842. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. V. Silva, S. Litovsky, J. A.R. Assad, A. L.S. Sousa, B. J. Martin, D. Vela, S. C. Coulter, J. Lin, J. Ober, W. K. Vaughn, et al. Mesenchymal Stem Cells Differentiate into an Endothelial Phenotype, Enhance Vascular Density, and Improve Heart Function in a Canine Chronic Ischemia Model Circulation, January 18, 2005; 111(2): 150 - 156. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. J. Rastan, T. Walther, M. Kostelka, J. Garbade, A. Schubert, A. Stein, S. Dhein, and F. W. Mohr Morphological, electrophysiological and coupling characteristics of bone marrow-derived mononuclear cells--an in vitro-model Eur. J. Cardiothorac. Surg., January 1, 2005; 27(1): 104 - 110. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Zhang, D. Wang, Z. Estrov, S. Raj, J. T. Willerson, and E. T.H. Yeh Both Cell Fusion and Transdifferentiation Account for the Transformation of Human Peripheral Blood CD34-Positive Cells Into Cardiomyocytes In Vivo Circulation, December 21, 2004; 110(25): 3803 - 3807. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. M. Regula, M. J. Rzeszutek, D. Baetz, C. Seneviratne, and L. A. Kirshenbaum Therapeutic opportunities for cell cycle re-entry and cardiac regeneration Cardiovasc Res, December 1, 2004; 64(3): 395 - 401. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Kawada, J. Fujita, K. Kinjo, Y. Matsuzaki, M. Tsuma, H. Miyatake, Y. Muguruma, K. Tsuboi, Y. Itabashi, Y. Ikeda, et al. Nonhematopoietic mesenchymal stem cells can be mobilized and differentiate into cardiomyocytes after myocardial infarction Blood, December 1, 2004; 104(12): 3581 - 3587. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Xu, M. Wani, Y.-S. Dai, J. Wang, M. Yan, A. Ayub, and M. Ashraf Differentiation of Bone Marrow Stromal Cells Into the Cardiac Phenotype Requires Intercellular Communication With Myocytes Circulation, October 26, 2004; 110(17): 2658 - 2665. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Matsuura, H. Wada, T. Nagai, Y. Iijima, T. Minamino, M. Sano, H. Akazawa, J. D. Molkentin, H. Kasanuki, and I. Komuro Cardiomyocytes fuse with surrounding noncardiomyocytes and reenter the cell cycle J. Cell Biol., October 25, 2004; 167(2): 351 - 363. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Schachinger, B. Assmus, M. B. Britten, J. Honold, R. Lehmann, C. Teupe, N. D. Abolmaali, T. J. Vogl, W.-K. Hofmann, H. Martin, et al. Transplantation of progenitor cells and regeneration enhancement in acute myocardial infarction: Final one-year results of the TOPCARE-AMI Trial J. Am. Coll. Cardiol., October 19, 2004; 44(8): 1690 - 1699. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. C. Perin, H. F.R. Dohmann, R. Borojevic, S. A. Silva, A. L.S. Sousa, G. V. Silva, C. T. Mesquita, L. Belem, W. K. Vaughn, F. O.D. Rangel, et al. Improved Exercise Capacity and Ischemia 6 and 12 Months After Transendocardial Injection of Autologous Bone Marrow Mononuclear Cells for Ischemic Cardiomyopathy Circulation, September 14, 2004; 110(11_suppl_1): II-213 - II-218. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Bani-Yaghoub, S. E. Kendall, D. P. Moore, S. Bellum, R. A. Cowling, G. N. Nikopoulos, C. J. Kubu, C. Vary, and J. M. Verdi Insulin acts as a myogenic differentiation signal for neural stem cells with multilineage differentiation potential Development, September 1, 2004; 131(17): 4287 - 4298. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. L. March and B. H. Johnstone Cellular approaches to tissue repair in cardiovascular disease: the more we know, the more there is to learn Am J Physiol Heart Circ Physiol, August 1, 2004; 287(2): H458 - H463. [Full Text] [PDF]
|
|
|
|
|
|
X. Wu, E. Rabkin-Aikawa, K. J. Guleserian, T. E. Perry, Y. Masuda, F. W. H. Sutherland, F. J. Schoen, J. E. Mayer Jr., and J. Bischoff Tissue-engineered microvessels on three-dimensional biodegradable scaffolds using human endothelial progenitor cells Am J Physiol Heart Circ Physiol, August 1, 2004; 287(2): H480 - H487. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. S. Lee and R. R. Makkar Stem-Cell Transplantation in Myocardial Infarction: A Status Report Ann Intern Med, May 4, 2004; 140(9): 729 - 737. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Heeschen, R. Lehmann, J. Honold, B. Assmus, A. Aicher, D. H. Walter, H. Martin, A. M. Zeiher, and S. Dimmeler Profoundly Reduced Neovascularization Capacity of Bone Marrow Mononuclear Cells Derived From Patients With Chronic Ischemic Heart Disease Circulation, April 6, 2004; 109(13): 1615 - 1622. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. E. Stec Smart Gene Therapy for the Heart Hypertension, April 1, 2004; 43(4): 720 - 721. [Full Text] [PDF]
|
|
|
|
|
|
C.-H. Wang, N. Ciliberti, S.-H. Li, P. E. Szmitko, R. D. Weisel, P. W.M. Fedak, M. Al-Omran, W.-J. Cherng, R.-K. Li, W. L. Stanford, et al. Rosiglitazone Facilitates Angiogenic Progenitor Cell Differentiation Toward Endothelial Lineage: A New Paradigm in Glitazone Pleiotropy Circulation, March 23, 2004; 109(11): 1392 - 1400. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Xaymardan, L. Tang, L. Zagreda, B. Pallante, J. Zheng, J. L. Chazen, A. Chin, I. Duignan, P. Nahirney, S. Rafii, et al. Platelet-Derived Growth Factor-AB Promotes the Generation of Adult Bone Marrow-Derived Cardiac Myocytes Circ. Res., March 19, 2004; 94(5): e39 - e45. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Xaymardan, J. Zheng, I. Duignan, A. Chin, J. M. Holm, V. L.T. Ballard, and J. M. Edelberg Senescent Impairment in Synergistic Cytokine Pathways That Provide Rapid Cardioprotection in the Rat Heart J. Exp. Med., March 15, 2004; 199(6): 797 - 804. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M R Alison, R Poulsom, W R Otto, P Vig, M Brittan, N C Direkze, M Lovell, T C Fang, S L Preston, and N A Wright Recipes for adult stem cell plasticity: fusion cuisine or readymade? J. Clin. Pathol., February 1, 2004; 57(2): 113 - 120. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Skowasch, A. Jabs, R. Andrie, S. Dinkelbach, B. Luderitz, and G. Bauriedel Presence of bone-marrow- and neural-crest-derived cells in intimal hyperplasia at the time of clinical in-stent restenosis Cardiovasc Res, December 1, 2003; 60(3): 684 - 691. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Gulati, D. Jevremovic, T. E. Peterson, S. Chatterjee, V. Shah, R. G. Vile, and R. D. Simari Diverse Origin and Function of Cells With Endothelial Phenotype Obtained From Adult Human Blood Circ. Res., November 28, 2003; 93(11): 1023 - 1025. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M.B. Britten, N.D. Abolmaali, B. Assmus, R. Lehmann, J. Honold, J. Schmitt, T.J. Vogl, H. Martin, V. Schachinger, S. Dimmeler, et al. Infarct Remodeling After Intracoronary Progenitor Cell Treatment in Patients With Acute Myocardial Infarction (TOPCARE-AMI): Mechanistic Insights From Serial Contrast-Enhanced Magnetic Resonance Imaging Circulation, November 4, 2003; 108(18): 2212 - 2218. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. T.H. Yeh, S. Zhang, H. D. Wu, M. Korbling, J. T. Willerson, and Z. Estrov Transdifferentiation of Human Peripheral Blood CD34+-Enriched Cell Population Into Cardiomyocytes, Endothelial Cells, and Smooth Muscle Cells In Vivo Circulation, October 28, 2003; 108(17): 2070 - 2073. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Oh, S. B. Bradfute, T. D. Gallardo, T. Nakamura, V. Gaussin, Y. Mishina, J. Pocius, L. H. Michael, R. R. Behringer, D. J. Garry, et al. Cardiac progenitor cells from adult myocardium: Homing, differentiation, and fusion after infarction PNAS, October 14, 2003; 100(21): 12313 - 12318. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Pesce, A. Orlandi, M. G. Iachininoto, S. Straino, A. R. Torella, V. Rizzuti, G. Pompilio, G. Bonanno, G. Scambia, and M. C. Capogrossi Myoendothelial Differentiation of Human Umbilical Cord Blood-Derived Stem Cells in Ischemic Limb Tissues Circ. Res., September 5, 2003; 93 (5): e51 - e62. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. N. Olson and M. D. Schneider Sizing up the heart: development redux in disease Genes & Dev., August 15, 2003; 17(16): 1937 - 1956. [Full Text] [PDF]
|
|
|
|
|
|
P. E. Szmitko, P. W.M. Fedak, R. D. Weisel, D. J. Stewart, M. J.B. Kutryk, and S. Verma Endothelial Progenitor Cells: New Hope for a Broken Heart Circulation, June 24, 2003; 107(24): 3093 - 3100. [Full Text] [PDF]
|
|
|
|
|
|
E. C. Perin, H. F.R. Dohmann, R. Borojevic, S. A. Silva, A. L.S. Sousa, C. T. Mesquita, M. I.D. Rossi, A. C. Carvalho, H. S. Dutra, H. J.F. Dohmann, et al. Transendocardial, Autologous Bone Marrow Cell Transplantation for Severe, Chronic Ischemic Heart Failure Circulation, May 13, 2003; 107(18): 2294 - 2302. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Anversa, J. Kajstura, B. Nadal-Ginard, and A. Leri Primitive Cells and Tissue Regeneration Circ. Res., April 4, 2003; 92(6): 579 - 582. [Full Text] [PDF]
|
|