doi: 10.1161/hc0502.102964
(Circulation. 2002;105:621.)
© 2002 American Heart Association, Inc.
Basic Science Reports |
Dynamic Changes in Three-Dimensional Architecture and Vascular Volume of Transmural Coronary Microvasculature Between Diastolic- and Systolic-Arrested Rat Hearts
From the Department of Medical Engineering and Systems Cardiology (E.T., Y.O., T.K., F.S., T.M., M.G., F.K.) and Department of Anatomy (K.F.), Kawasaki Medical School, Okayama, Japan; and the Department of Physiology II (F.K.), Okayama University Medical School, Okayama, Japan.
Correspondence to Eiji Toyota, MD, PhD, Department of Physiology, Medical College of Wisconsin, 8701 Watertown Plank Rd, PO Box 26509, Milwaukee, WI 53226-0509 (e-mail etoyota{at}mcw.edu), and reprints requests to Fumihiko Kajiya, MD, PhD, Department of Medical Engineering and Systems Cardiology, Kawasaki Medical School, 577 Matsushima, Kurashiki, Okayama, 701-0192 Japan (e-mail kajiya@me.kawasaki-m.ac.jp).
Background— The phase difference of coronary arterial and venous flows indicates the importance of intramyocardial capacitance vessels in storing diastolic flow and in discharging volume in systole. However, the anatomic and functional characteristics of the capacitance vessels are unclear. We aimed to clarify those characteristics with their transmural difference by 3D visualization of transmural microvessels under diastole and systole.
Methods and Results— We performed complete intracoronary filling of a contrast medium into Langendorff’s Wistar rat hearts under (1) St Thomas–perfused diastolic arrest (D-mode) and (2) BaCl2-induced systolic arrest (S-mode). Precise transmural 3D architectures of capillaries and of pre- and post-capillary microvessels (ie, microvessels larger than capillaries) were visualized clearly with a confocal laser scanning microscope and x-ray microcomputed tomography (microCT), respectively. Vascular volume fraction (VF) and systolic-induced VF reduction rate from D- to S-mode were analyzed. The net capillary VF in D-mode (20.4±0.9%) was 10 times that of larger microvessels and was reduced in S-mode by 32% without capillary collapse. Systolic-induced VF reduction rate was smaller in capillaries than in larger microvessels (48%; P<0.05). The larger microvessel VF in D-mode (2.2±0.2%) was reduced in S-mode, accompanied by complicated 3D deformation.
Conclusions— Capillaries were relatively resistant to the systolic extravascular compression compared with pre- and post-capillary microvessels, conveniently beneficial for the myocardial oxygen delivery throughout a cardiac cycle. Nevertheless, a larger change in the absolute volume of capillaries may function as effective capacitance. On one hand, the pre- and post-capillary microvessels showed a larger phasic change in resistance, which may function to maintain the capillary patency during systole.
Key Words: microcirculation • imaging • myocardial contraction
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