doi: 10.1161/hc4501.098944
(Circulation. 2001;104:2492.)
© 2001 American Heart Association, Inc.
Basic Science Reports |
Mitochondrial Abnormalities in Tumor Necrosis Factor-
–Induced Heart Failure Are Associated With Impaired DNA Repair Activity
From the Cardiovascular Institute (Y.Y.L., A.M.F.) and the Center for Biological Imaging (S.C.W.), University of Pittsburgh School of Medicine, Pittsburgh, Pa; and Dow Neurobiology Laboratories, Legacy Research, Portland, Ore (D.C.).
Correspondence to Arthur M. Feldman, MD, PhD, Cardiovascular Institute, University of Pittsburgh School of Medicine, 572 Scaife Hall, 200 Lothrop St, Pittsburgh, PA 15213. E-mail feldmanam{at}msx.upmc.edu
Background— Recent studies suggest that mutations in cardiac mitochondrial DNA (mtDNA) may contribute to the development of dilated cardiomyopathy. The mechanisms that regulate those mutations, however, remain undefined. Thus, we studied cardiac mtDNA repair mechanisms, mtDNA damage, and mitochondrial structure and function in mice with heart failure secondary to overexpression of TNF-
(TNF1.6 mice).
Methods and Results— We studied mtDNA repair by measuring the uracil DNA glycosylase (mtUDG) and base excision repair activities. mtDNA damage was assessed by Southern blot of Fpg protein–digested mtDNA. Mitochondrial ultrastructural changes were examined by electron microscopy, and function by cytochrome c oxidase and succinate dehydrogenase activity assays. The results showed that both mtUDG and base excision repair activities were significantly reduced in TNF1.6 mouse heart. Fpg-sensitive sites were markedly increased in TNF1.6 mouse cardiac mtDNA, suggesting increased mtDNA damage. Mitochondrial function as demonstrated by cardiac cytochrome c oxidase activity was also markedly reduced. Cardiac ATP content was not changed, however, suggesting a shift from oxidative phosphorylation to glycolysis, as shown by increased LDH and ALT activities and lactate/pyruvate ratio. Ultrastructurally, the TNF1.6 mouse cardiac mitochondria became irregular in shape and smaller, and the cristae were decreased and appeared disorganized, with breaks.
Conclusions— These results suggest that mtDNA mutations and mitochondrial structural and functional alterations in TNF-–induced heart failure may be associated with reduced mtDNA repair activity, and the pathophysiological effects of TNF- on the heart may be mediated, at least in part, through these changes in mitochondria.
Key Words: heart failure • molecular biology • metabolism • structure
This article has been cited by other articles:
 |
|
 |
|
  X. Palomer, D. Alvarez-Guardia, R. Rodriguez-Calvo, T. Coll, J. C. Laguna, M. M. Davidson, T. O. Chan, A. M. Feldman, and M. Vazquez-Carrera TNF-{alpha} reduces PGC-1{alpha} expression through NF-{kappa}B and p38 MAPK leading to increased glucose oxidation in a human cardiac cell model Cardiovasc Res, March 1, 2009; 81(4): 703 - 712. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Tsutsui, S. Kinugawa, and S. Matsushima Mitochondrial oxidative stress and dysfunction in myocardial remodelling Cardiovasc Res, February 15, 2009; 81(3): 449 - 456. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Panagopoulou, C. H. Davos, D. J. Milner, E. Varela, J. Cameron, D. L. Mann, and Y. Capetanaki Desmin mediates TNF-{alpha}-induced aggregate formation and intercalated disk reorganization in heart failure J. Cell Biol., October 20, 2008; 181(5): 761 - 775. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. C. Sparagna, A. J. Chicco, R. C. Murphy, M. R. Bristow, C. A. Johnson, M. L. Rees, M. L. Maxey, S. A. McCune, and R. L. Moore Loss of cardiac tetralinoleoyl cardiolipin in human and experimental heart failure J. Lipid Res., July 1, 2007; 48(7): 1559 - 1570. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. R. Bergman, J. R. Teerlink, R. Mahimkar, L. Li, B.-Q. Zhu, A. Nguyen, S. Dahi, J. S. Karliner, and D. H. Lovett Cardiac matrix metalloproteinase-2 expression independently induces marked ventricular remodeling and systolic dysfunction Am J Physiol Heart Circ Physiol, April 1, 2007; 292(4): H1847 - H1860. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Nisoli, E. Clementi, M. O. Carruba, and S. Moncada Defective Mitochondrial Biogenesis: A Hallmark of the High Cardiovascular Risk in the Metabolic Syndrome? Circ. Res., March 30, 2007; 100(6): 795 - 806. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Ojaimi, W. Li, S. Kinugawa, H. Post, A. Csiszar, P. Pacher, G. Kaley, and T. H. Hintze Transcriptional basis for exercise limitation in male eNOS-knockout mice with age: heart failure and the fetal phenotype Am J Physiol Heart Circ Physiol, October 1, 2005; 289(4): H1399 - H1407. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A Garnier, D Fortin, C Delomenie, I Momken, V Veksler, and R Ventura-Clapier Depressed mitochondrial transcription factors and oxidative capacity in rat failing cardiac and skeletal muscles J. Physiol., September 1, 2003; 551(2): 491 - 501. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Suematsu, H. Tsutsui, J. Wen, D. Kang, M. Ikeuchi, T. Ide, S. Hayashidani, T. Shiomi, T. Kubota, N. Hamasaki, et al. Oxidative Stress Mediates Tumor Necrosis Factor-{alpha}-Induced Mitochondrial DNA Damage and Dysfunction in Cardiac Myocytes Circulation, March 18, 2003; 107(10): 1418 - 1423. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Machida, T. Kubota, N. Kawamura, H. Funakoshi, T. Ide, H. Utsumi, Y. Y. Li, A. M. Feldman, H. Tsutsui, H. Shimokawa, et al. Overexpression of tumor necrosis factor-alpha increases production of hydroxyl radical in murine myocardium Am J Physiol Heart Circ Physiol, February 1, 2003; 284(2): H449 - H455. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. KARAHALIL, B. A. HOGUE, N. C. DE SOUZA-PINTO, and V. A. BOHR Base excision repair capacity in mitochondria and nuclei: tissue-specific variations FASEB J, December 1, 2002; 16(14): 1895 - 1902. [Abstract] [Full Text] [PDF]
|
|