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(Circulation. 2001;104:1850.)
© 2001 American Heart Association, Inc.

Basic Science Reports

Effect of Short Pulsed Nonablative Infrared Laser Irradiation on Vascular Cells In Vitro and Neointimal Hyperplasia in a Rabbit Balloon Injury Model

Nicholas Kipshidze, MD PhD; Victor Nikolaychik, MD PhD; Michael Muckerheidi; Michael H. Keelan, MD; Valeri Chekanov, MD PhD; Michelle Maternowski, BS; Paramjit Chawla, MD; Irina Hernandez, MD; Sriram Iyer, MD; George Dangas, MD PhD; Harry Sahota, MD; Martin B. Leon, MD; Gary Roubin, MD PhD; Jeffrey W. Moses, MD

From Lenox Hill Heart and Vascular Institute of New York and Cardiovascular Research Foundation, New York, NY (N.K., S.I., G.D., M.B.L., G.R., J.W.M.); Milwaukee Heart Institute, Milwaukee (V.N., V.C., M. Maternowski, I.H.), Alien Technologies, Port Washington (M. Muckerheidi), and Medical College of Wisconsin, Milwaukee (M.H.K., P.C.), Wis; and Good Samaritan Hospital, Los Angeles, Calif (H.S.).

Correspondence to Nicholas Kipshidze, MD, PhD, Lenox Hill Heart and Vascular Institute, 130 E 77th St, Black Hall, 9th Floor, New York, NY 10021. E-mail nkipshidze{at}lenoxhill.net

Background— Neointimal hyperplasia after PTCA is an important component of restenosis.

Methods and Results— Cultures of rabbit endothelial cells and smooth muscle cells (SMCs) were irradiated with different doses of nonablative infrared (1064-nm) radiation. Normalized viability index detected with nondestructive Alamar Blue assay and direct cell count were studied. Our experiments demonstrated dose-dependent cytostatic or cytotoxic effects of laser irradiation. We also evaluated the long-term effect of endoluminal nonablative infrared laser irradiation on neointimal hyperplasia in a rabbit balloon injury model. PTCA of both iliac arteries of 23 New Zealand White rabbits was performed. One iliac artery was subjected to intra-arterial subablative infrared irradiation via a diffuse tip fiber. The contralateral vessel served as control. The diet was supplemented with 0.25% cholesterol and 2% peanut oil for 10 days before and 60 days after PTCA. Morphometry after 60 days showed that intimal areas were 0.76±0.18 and 1.85±0.30 mm² in the laser and control arteries, respectively (P=2.2x10^-11).

Conclusions— We conclude that nonablative infrared laser inhibited neointimal hyperplasia after PTCA in cholesterol-fed rabbits for up to 60 days.

Key Words: lasers • angioplasty • restenosis • muscle, smooth • cells

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