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(Circulation. 2000;102:2165.)
© 2000 American Heart Association, Inc.
Brief Rapid Communications |
Direct Proinflammatory Effect of C-Reactive Protein on Human Endothelial Cells
From the Department of Cardiology (E.T.H.Y.), University of Texas–M.D. Anderson Cancer Center, Houston, Tex; Department of Internal Medicine (V.P., J.T.W., E.T.H.Y.) and Institute of Molecular Medicine for the Prevention of Human Diseases (E.T.H.Y.), University of Texas Health Science Center, Houston, Tex; and the Texas Heart Institute (V.P., J.T.W., E.T.H.Y.), St. Luke’s Episcopal Hospital, Houston, Tex.
Correspondence to Edward T.H. Yeh, MD, Department of Cardiology, 1515 Holcombe Blvd, Box 70, University of Texas–M.D. Anderson Cancer Center, Houston, TX 77030-4095.
Background—The acute-phase reactant C-reactive protein (CRP) is an important risk factor for coronary heart disease. However, the possible effects of CRP on vascular cells are not known.
Methods and Results—We tested the effects of CRP on expression of
adhesion molecules in both human umbilical vein and coronary
artery endothelial cells. Expression of vascular cell
adhesion molecule (VCAM-1), intercellular adhesion molecule (ICAM-1),
and E-selectin was assessed by flow cytometry. Incubation with
recombinant human CRP (10 µg/mL) for 24 hours induced an 
10-fold
increase in expression of ICAM-1 and a significant expression of
VCAM-1, whereas a 6-hour incubation induced significant E-selectin
expression. Adhesion molecule induction was similar to that observed in
endothelial cells activated with
interleukin-1ß. In coronary artery
endothelial cells, induction of ICAM-1 and VCAM-1 was
already present at 5 µg/mL and reached a maximum at 50 µg/mL,
at which point a substantial increase in expression of E-selectin was
also evident. The CRP effect was dependent on presence of human serum
in the culture medium, because no effect was seen in cells cultured
with serum-free medium. In contrast, interleukin-1ß was able to
induce adhesion molecule expression in the absence of human serum.
Conclusions—CRP induces adhesion molecule expression in human endothelial cells in the presence of serum. These findings support the hypothesis that CRP may play a direct role in promoting the inflammatory component of atherosclerosis and present a potential target for the treatment of atherosclerosis.
Key Words: cell adhesion molecules • endothelium • atherosclerosis
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