(Circulation. 2000;101:841.)
© 2000 American Heart Association, Inc.
Brief Rapid Communications |
Elevated Levels of Shed Membrane Microparticles With Procoagulant Potential in the Peripheral Circulating Blood of Patients With Acute Coronary Syndromes
From the Institut National de la Santé et la Recherche Médicale, INSERM U141, IFR Circulation, Hôpital Lariboisière, Paris (Z.M., A.T.); Service de Cardiologie (H.B., P.G.S.) and Service de Biochimie (J.B.), Hôpital Bichat, Paris; Institut d’Hématologie et d’Immunologie, Faculté de Médecine, Université Louis Pasteur, Strasbourg; and INSERM U143, Le Kremlin-Bicêtre (B.H., J.-M.F.), France.
Correspondence to Alain Tedgui, PhD, INSERM U 141, 41 boulevard de la Chapelle, 75475 Paris Cedex 10, France. E-mail tedgui{at}infobiogen.fr
Background—Apoptotic microparticles are responsible for almost all tissue factor activity of the plaque lipid core. We hypothesized that elevated levels of procoagulant microparticles could also circulate in the peripheral blood of patients with recent clinical signs of plaque disruption and thrombosis.
Methods and Results—We studied 39 patients with coronary heart disease, including 12 patients with stable angina and 27 patients with acute coronary syndromes (ACS), and 12 patients with noncoronary heart disease. We isolated the circulating microparticles by capture with annexin V and determined their procoagulant potential with a prothrombinase assay. The cell origins of microparticles were determined in an additional 22 patients by antigenic capture with specific antibodies. The level of procoagulant microparticles did not differ between stable angina patients and noncoronary patients (10.1±1.6 nmol/L phosphatidylserine [PS] equivalent versus 9.9±1.6 nmol/L PS equivalent, respectively). However, procoagulant microparticles were significantly elevated in patients with ACS (22.2±2.7 nmol/L PS equivalent) compared with other coronary (P<0.01) or noncoronary (P<0.01) patients. Microparticles of endothelial origin were significantly elevated in patients with ACS (P<0.01), which suggests an important role for endothelial injury in inducing the procoagulant potential.
Conclusions—High levels of procoagulant endothelial microparticles are present in the circulating blood of patients with ACS and may contribute to the generation and perpetuation of intracoronary thrombi.
Key Words: atherosclerosis • complications • thrombosis • microparticles • endothelium.
This article has been cited by other articles:
 |
|
 |
|
  D J Hausenloy and D M Yellon Targeting residual cardiovascular risk: raising high-density lipoprotein cholesterol levels Heart, June 1, 2008; 94(6): 706 - 714. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Habib, C. Kunzelmann, W. Shamseddeen, F. Zobairi, J.-M. Freyssinet, and A. Taher Elevated levels of circulating procoagulant microparticles in patients with {beta}-thalassemia intermedia Haematologica, June 1, 2008; 93(6): 941 - 942. [Full Text] [PDF]
|
|
|
|
|
|
S. D. Turco, G. Basta, G. Lazzerini, M. Evangelista, G. Rainaldi, P. Tanganelli, J. H. Christensen, E. B. Schmidt, and R. De Caterina Effect of the administration of n-3 polyunsaturated fatty acids on circulating levels of microparticles in patients with a previous myocardial infarction Haematologica, June 1, 2008; 93(6): 892 - 899. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. A. Mostefai, A. Agouni, N. Carusio, M. L. Mastronardi, C. Heymes, D. Henrion, R. Andriantsitohaina, and M. C. Martinez Phosphatidylinositol 3-Kinase and Xanthine Oxidase Regulate Nitric Oxide and Reactive Oxygen Species Productions by Apoptotic Lymphocyte Microparticles in Endothelial Cells J. Immunol., April 1, 2008; 180(7): 5028 - 5035. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Schouten, W. J. Wiersinga, M. Levi, and T. van der Poll Inflammation, endothelium, and coagulation in sepsis J. Leukoc. Biol., March 1, 2008; 83(3): 536 - 545. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Tesse, G. Al-Massarani, R. Wangensteen, S. Reitenbach, M. C. Martinez, and R. Andriantsitohaina Rosiglitazone, a Peroxisome Proliferator-Activated Receptor-{gamma} Agonist, Prevents Microparticle-Induced Vascular Hyporeactivity through the Regulation of Proinflammatory Proteins J. Pharmacol. Exp. Ther., February 1, 2008; 324(2): 539 - 547. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. M. Heikkila, S. Latti, M. J. Leskinen, J. K. Hakala, P. T. Kovanen, and K. A. Lindstedt Activated Mast Cells Induce Endothelial Cell Apoptosis by a Combined Action of Chymase and Tumor Necrosis Factor-{alpha} Arterioscler. Thromb. Vasc. Biol., February 1, 2008; 28(2): 309 - 314. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Yang, B. R. Mwaikambo, T. Zhu, C. Gagnon, J. Lafleur, S. Seshadri, P. Lachapelle, J.-C. Lavoie, S. Chemtob, and P. Hardy Lymphocytic microparticles inhibit angiogenesis by stimulating oxidative stress and negatively regulating VEGF-induced pathways Am J Physiol Regulatory Integrative Comp Physiol, February 1, 2008; 294(2): R467 - R476. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Lu, W. Jiang, J.-H. Yang, P.-Y. Chang, J. P. Walterscheid, H.-H. Chen, M. Marcelli, D. Tang, Y.-T. Lee, W. S.L. Liao, et al. Electronegative LDL Impairs Vascular Endothelial Cell Integrity in Diabetes by Disrupting Fibroblast Growth Factor 2 (FGF2) Autoregulation Diabetes, January 1, 2008; 57(1): 158 - 166. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Salanova, M. Choi, S. Rolle, M. Wellner, F. C. Luft, and R. Kettritz beta2-Integrins and Acquired Glycoprotein IIb/IIIa (GPIIb/IIIa) Receptors Cooperate in NF-{kappa}B Activation of Human Neutrophils J. Biol. Chem., September 21, 2007; 282(38): 27960 - 27969. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Agouni, H. A. Mostefai, C. Porro, N. Carusio, J. Favre, V. Richard, D. Henrion, M. C. Martinez, and R. Andriantsitohaina Sonic hedgehog carried by microparticles corrects endothelial injury through nitric oxide release FASEB J, September 1, 2007; 21(11): 2735 - 2741. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. M. Aliotta, F. M. Sanchez-Guijo, G. J. Dooner, K. W. Johnson, M. S. Dooner, K. A. Greer, D. Greer, J. Pimentel, L. M. Kolankiewicz, N. Puente, et al. Alteration of Marrow Cell Gene Expression, Protein Production, and Engraftment into Lung by Lung-Derived Microvesicles: A Novel Mechanism for Phenotype Modulation Stem Cells, September 1, 2007; 25(9): 2245 - 2256. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Wang, Y. Zheng, W. Zhang, H. Yu, K. Lou, Y. Zhang, Q. Qin, B. Zhao, Y. Yang, and R. Hui Polymorphisms of KDR Gene Are Associated With Coronary Heart Disease J. Am. Coll. Cardiol., August 21, 2007; 50(8): 760 - 767. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Baroni, C. Pizzirani, M. Pinotti, D. Ferrari, E. Adinolfi, S. Calzavarini, P. Caruso, F. Bernardi, and F. Di Virgilio Stimulation of P2 (P2X7) receptors in human dendritic cells induces the release of tissue factor-bearing microparticles FASEB J, June 1, 2007; 21(8): 1926 - 1933. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. A. El Solh, M. E. Akinnusi, F. H. Baddoura, and C. R. Mankowski Endothelial Cell Apoptosis in Obstructive Sleep Apnea: A Link to Endothelial Dysfunction Am. J. Respir. Crit. Care Med., June 1, 2007; 175(11): 1186 - 1191. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Lerman, D. R. Holmes, J. Herrmann, and B. J. Gersh Microcirculatory dysfunction in ST-elevation myocardial infarction: cause, consequence, or both? Eur. Heart J., April 1, 2007; 28(7): 788 - 797. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. E. Deanfield, J. P. Halcox, and T. J. Rabelink Endothelial Function and Dysfunction: Testing and Clinical Relevance Circulation, March 13, 2007; 115(10): 1285 - 1295. [Full Text] [PDF]
|
|
|
|
 |
|
 B. Szotowski, S. Antoniak, P. Goldin-Lang, Q.-V. Tran, K. Pels, P. Rosenthal, V. Y. Bogdanov, H.-H. Borchert, H.-P. Schultheiss, and U. Rauch Antioxidative treatment inhibits the release of thrombogenic tissue factor from irradiation- and cytokine-induced endothelial cells Cardiovasc Res, March 1, 2007; 73(4): 806 - 812. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. H. Edmunds Jr and R. W. Colman Thrombin During Cardiopulmonary Bypass Ann. Thorac. Surg., December 1, 2006; 82(6): 2315 - 2322. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Morel, F. Toti, B. Hugel, B. Bakouboula, L. Camoin-Jau, F. Dignat-George, and J.-M. Freyssinet Procoagulant Microparticles: Disrupting the Vascular Homeostasis Equation? Arterioscler. Thromb. Vasc. Biol., December 1, 2006; 26(12): 2594 - 2604. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Chironi, A. Simon, B. Hugel, M. Del Pino, J. Gariepy, J.-M. Freyssinet, and A. Tedgui Circulating Leukocyte-Derived Microparticles Predict Subclinical Atherosclerosis Burden in Asymptomatic Subjects Arterioscler. Thromb. Vasc. Biol., December 1, 2006; 26(12): 2775 - 2780. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. C. Martinez, F. Larbret, F. Zobairi, J. Coulombe, N. Debili, W. Vainchenker, M. Ruat, and J.-M. Freyssinet Transfer of differentiation signal by membrane microvesicles harboring hedgehog morphogens Blood, November 1, 2006; 108(9): 3012 - 3020. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. V. Mitsios, M. P. Vini, D. Stengel, E. Ninio, and A. D. Tselepis Human Platelets Secrete the Plasma Type of Platelet-Activating Factor Acetylhydrolase Primarily Associated With Microparticles Arterioscler. Thromb. Vasc. Biol., August 1, 2006; 26(8): 1907 - 1913. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. M. Boulanger, N. Amabile, and A. Tedgui Circulating Microparticles: A Potential Prognostic Marker for Atherosclerotic Vascular Disease Hypertension, August 1, 2006; 48(2): 180 - 186. [Full Text] [PDF]
|
|
|
|
 |
|
 C. Mineo, H. Deguchi, J. H. Griffin, and P. W. Shaul Endothelial and Antithrombotic Actions of HDL Circ. Res., June 9, 2006; 98(11): 1352 - 1364. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-J. Stampfuss, P. Censarek, J. W. Fischer, K. Schror, and A.-A. Weber Rapid Release of Active Tissue Factor From Human Arterial Smooth Muscle Cells Under Flow Conditions Arterioscler. Thromb. Vasc. Biol., May 1, 2006; 26(5): e34 - e37. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Tedgui and Z. Mallat Cytokines in Atherosclerosis: Pathogenic and Regulatory Pathways Physiol Rev, April 1, 2006; 86(2): 515 - 581. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Steffel, T. F. Luscher, and F. C. Tanner Tissue Factor in Cardiovascular Diseases: Molecular Mechanisms and Clinical Implications Circulation, February 7, 2006; 113(5): 722 - 731. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Werner, S. Wassmann, P. Ahlers, S. Kosiol, and G. Nickenig Circulating CD31+/Annexin V+ Apoptotic Microparticles Correlate With Coronary Endothelial Function in Patients With Coronary Artery Disease Arterioscler. Thromb. Vasc. Biol., January 1, 2006; 26(1): 112 - 116. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Herrmann Peri-procedural myocardial injury: 2005 update Eur. Heart J., December 1, 2005; 26(23): 2493 - 2519. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Tesse, M. C. Martinez, B. Hugel, K. Chalupsky, C. D. Muller, F. Meziani, D. Mitolo-Chieppa, J.-M. Freyssinet, and R. Andriantsitohaina Upregulation of Proinflammatory Proteins Through NF-{kappa}B Pathway by Shed Membrane Microparticles Results in Vascular Hyporeactivity Arterioscler. Thromb. Vasc. Biol., December 1, 2005; 25(12): 2522 - 2527. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Malarstig, T. Tenno, N. Johnston, B. Lagerqvist, T. Axelsson, A.-C. Syvanen, L. Wallentin, and A. Siegbahn Genetic Variations in the Tissue Factor Gene Are Associated With Clinical Outcome in Acute Coronary Syndrome and Expression Levels in Human Monocytes Arterioscler. Thromb. Vasc. Biol., December 1, 2005; 25(12): 2667 - 2672. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Mallat, Ph. G. Steg, J. Benessiano, M.-L. Tanguy, K. A. Fox, J.-P. Collet, O. H. Dabbous, P. Henry, K. F. Carruthers, A. Dauphin, et al. Circulating Secretory Phospholipase A2 Activity Predicts Recurrent Events in Patients With Severe Acute Coronary Syndromes J. Am. Coll. Cardiol., October 4, 2005; 46(7): 1249 - 1257. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Mezentsev, R. M. H. Merks, E. O'Riordan, J. Chen, N. Mendelev, M. S. Goligorsky, and S. V. Brodsky Endothelial microparticles affect angiogenesis in vitro: role of oxidative stress Am J Physiol Heart Circ Physiol, September 1, 2005; 289(3): H1106 - H1114. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Hattori, M. M.H. Khan, R. W. Colman, and L. H. Edmunds Jr Plasma Tissue Factor Plus Activated Peripheral Mononuclear Cells Activate Factors VII and X in Cardiac Surgical Wounds J. Am. Coll. Cardiol., August 16, 2005; 46(4): 707 - 713. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. M. Boulanger and A. Tedgui Dying for attention: Microparticles and angiogenesis Cardiovasc Res, July 1, 2005; 67(1): 1 - 3. [Full Text] [PDF]
|
|
|
|
|
|
I. Ott Soluble Tissue Factor Emerges From Inflammation Circ. Res., June 24, 2005; 96(12): 1217 - 1218. [Full Text] [PDF]
|
|
|
|
|
|
H. Koga, S. Sugiyama, K. Kugiyama, K. Watanabe, H. Fukushima, T. Tanaka, T. Sakamoto, M. Yoshimura, H. Jinnouchi, and H. Ogawa Elevated Levels of VE-Cadherin-Positive Endothelial Microparticles in Patients With Type 2 Diabetes Mellitus and Coronary Artery Disease J. Am. Coll. Cardiol., May 17, 2005; 45(10): 1622 - 1630. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Llorente-Cortes and L. Badimon LDL Receptor-Related Protein and the Vascular Wall: Implications for Atherothrombosis Arterioscler. Thromb. Vasc. Biol., March 1, 2005; 25(3): 497 - 504. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. C. Martinez, A. Tesse, F. Zobairi, and R. Andriantsitohaina Shed membrane microparticles from circulating and vascular cells in regulating vascular function Am J Physiol Heart Circ Physiol, March 1, 2005; 288(3): H1004 - H1009. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. L. Yu, L. May, V. Lhotak, S. Shahrzad, S. Shirasawa, J. I. Weitz, B. L. Coomber, N. Mackman, and J. W. Rak Oncogenic events regulate tissue factor expression in colorectal cancer cells: implications for tumor progression and angiogenesis Blood, February 15, 2005; 105(4): 1734 - 1741. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Busch, I. Seitz, B. Steppich, S. Hess, R. Eckl, A. Schomig, and I. Ott Coagulation Factor Xa Stimulates Interleukin-8 Release in Endothelial Cells and Mononuclear Leukocytes: Implications in Acute Myocardial Infarction Arterioscler. Thromb. Vasc. Biol., February 1, 2005; 25(2): 461 - 466. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. M. Day, J. L. Reeve, B. Pedersen, D. M Farris, D. D. Myers, M. Im, T. W. Wakefield, N. Mackman, and W. P. Fay Macrovascular thrombosis is driven by tissue factor derived primarily from the blood vessel wall Blood, January 1, 2005; 105(1): 192 - 198. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. R. Moreno and V. Fuster New aspects in the pathogenesis of diabetic atherothrombosis J. Am. Coll. Cardiol., December 21, 2004; 44(12): 2293 - 2300. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. C. Ferreira, A. A. Peter, A. J. Mendez, J. J. Jimenez, L. M. Mauro, J. A. Chirinos, R. Ghany, S. Virani, S. Garcia, L. L. Horstman, et al. Postprandial Hypertriglyceridemia Increases Circulating Levels of Endothelial Cell Microparticles Circulation, December 7, 2004; 110(23): 3599 - 3603. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Hristov, W. Erl, S. Linder, and P. C. Weber Apoptotic bodies from endothelial cells enhance the number and initiate the differentiation of human endothelial progenitor cells in vitro Blood, November 1, 2004; 104(9): 2761 - 2766. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. G. Birukov, V. N. Bochkov, A. A. Birukova, K. Kawkitinarong, A. Rios, A. Leitner, A. D. Verin, G. M. Bokoch, N. Leitinger, and Joe. G.N. Garcia Epoxycyclopentenone-Containing Oxidized Phospholipids Restore Endothelial Barrier Function via Cdc42 and Rac Circ. Res., October 29, 2004; 95(9): 892 - 901. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Gasser and J. A. Schifferli Activated polymorphonuclear neutrophils disseminate anti-inflammatory microparticles by ectocytosis Blood, October 15, 2004; 104(8): 2543 - 2548. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Llorente-Cortes, M. Otero-Vinas, S. Camino-Lopez, O. Llampayas, and L. Badimon Aggregated Low-Density Lipoprotein Uptake Induces Membrane Tissue Factor Procoagulant Activity and Microparticle Release in Human Vascular Smooth Muscle Cells Circulation, July 27, 2004; 110(4): 452 - 459. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Aras, A. Shet, R. R. Bach, J. L. Hysjulien, A. Slungaard, R. P. Hebbel, G. Escolar, B. Jilma, and N. S. Key Induction of microparticle- and cell-associated intravascular tissue factor in human endotoxemia Blood, June 15, 2004; 103(12): 4545 - 4553. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Suhara, K. Fukuo, O. Yasuda, M. Tsubakimoto, Y. Takemura, H. Kawamoto, T. Yokoi, M. Mogi, T. Kaimoto, and T. Ogihara Homocysteine Enhances Endothelial Apoptosis via Upregulation of Fas-Mediated Pathways Hypertension, June 1, 2004; 43(6): 1208 - 1213. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Durand, A. Scoazec, A. Lafont, J. Boddaert, A. Al Hajzen, F. Addad, M. Mirshahi, M. Desnos, A. Tedgui, and Z. Mallat In Vivo Induction of Endothelial Apoptosis Leads to Vessel Thrombosis and Endothelial Denudation: A Clue to the Understanding of the Mechanisms of Thrombotic Plaque Erosion Circulation, June 1, 2004; 109(21): 2503 - 2506. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Rajagopalan, E. C. Somers, R. D. Brook, C. Kehrer, D. Pfenninger, E. Lewis, A. Chakrabarti, B. C. Richardson, E. Shelden, W. J. McCune, et al. Endothelial cell apoptosis in systemic lupus erythematosus: a common pathway for abnormal vascular function and thrombosis propensity Blood, May 15, 2004; 103(10): 3677 - 3683. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Aprahamian, I. Rifkin, R. Bonegio, B. Hugel, J.-M. Freyssinet, K. Sato, J. J. Castellot Jr., and K. Walsh Impaired Clearance of Apoptotic Cells Promotes Synergy between Atherogenesis and Autoimmune Disease J. Exp. Med., April 19, 2004; 199(8): 1121 - 1131. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Martin, A. Tesse, B. Hugel, M. C. Martinez, O. Morel, J.-M. Freyssinet, and R. Andriantsitohaina Shed Membrane Particles From T Lymphocytes Impair Endothelial Function and Regulate Endothelial Protein Expression Circulation, April 6, 2004; 109(13): 1653 - 1659. [Abstract] [Full Text] [PDF]
|
|
|
|