(Circulation. 2000;101:1172.)
© 2000 American Heart Association, Inc.
Basic Science Reports |
The Cardiac Fas (APO-1/CD95) Receptor/Fas Ligand System
Relation to Diastolic Wall Stress in Volume-Overload Hypertrophy In Vivo and Activation of the Transcription Factor AP-1 in Cardiac Myocytes
From the Department of Cardiology and Angiology (K.C.W., J.H., M.L., B.F., H.D.) and the Department of Anatomy (J.W.), Medizinische Hochschule Hannover, Hannover, the Department of Internal Medicine I, University of Tübingen (M.K.A.B., K.S.-O.), Tübingen, Germany, and the Department of Cardiovascular Research, Ciba-Geigy, Basel, Switzerland (W.Z., D.L.).
Correspondence to Helmut Drexler, MD, Department of Cardiology and Angiology, Medizinische Hochschule Hannover, Carl-Neuberg-Str 1, 30625 Hannover, Germany. E-mail drexler.helmut{at}mh-hannover.de
Background—Fas (APO-1/CD95) is a
transmembrane receptor belonging to the tumor necrosis factor receptor
superfamily. Cross-linking of Fas by Fas ligand (FasL), a tumor
necrosis factor-
–related cytokine, promotes
apoptosis and/or transcription factor activation in a highly
cell-type–specific manner. The biological consequences of Fas
activation in cardiomyocytes and the regulation of Fas and
FasL abundance in the myocardium in vivo remain
largely unknown.
Methods and Results—As shown by immunohistochemistry, Fas was expressed on the sarcolemma of cardiomyocytes in left ventricular tissue sections. Moreover, FasL was constitutively expressed in the myocardium and in isolated cardiomyocytes, as revealed by reverse transcription polymerase chain reaction and Western blotting. Left ventricular abundance of Fas but not FasL was upregulated in a rat model of compensated volume-overload hypertrophy and was closely related to diastolic but not systolic wall stress as determined by MRI. Cardiomyocyte apoptosis was not enhanced in volume-overload hypertrophy despite the increased expression of Fas and the presence of FasL in the myocardium. Moreover, injection of mice with an agonistic anti-Fas antibody promoted hepatocyte but not cardiomyocyte apoptosis in vivo. Stimulation of isolated cardiomyocytes with recombinant FasL promoted an activation of the transcription factor AP-1 as shown by electrophoretic mobility shift assays but did not induce cell death.
Conclusions—Fas and FasL are constitutively expressed in the myocardium and in cardiomyocytes. Myocardial expression of Fas is closely related to diastolic loading conditions in vivo. Signaling pathways emanating from Fas are coupled to an activation of the transcription factor AP-1 in cardiomyocytes.
Key Words: receptors • myocytes • apoptosis • hypertrophy
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