Files in this Data Supplement:

• Figure I - (TIF file) (210 KB). Comparative TLR expression in postmortem and surgical artery samples. mRNA was isolated from 4 aorta and 4 carotid artery specimens obtained during surgery and processed immediately as described above. Alternatively, carotid and aorta specimens were collected at postmortem examination. Only sections free of atherosclerosis and inflammatory infiltrates were used. TLR expression for each member of the TLR family was quantified by RT-PCR in the surgical samples (gray bars) and in the autopsy tissues (black bars). Two-way ANOVA revealed no significant difference in TLR expression between postmortem and surgical samples both for aorta and carotid artery.
• Figure II - (TIF file) (1.3 MB). Postmortem stability of TLR profile in aortic wall tissue. Autopsy was performed 5 hours after death. Aorta was either immediately processed as described or kept at 4°C in 5%penicillin/streptomycin PBS for 30 hours. Total RNA was simultaneously isolated from samples shock-frozen at the indicated time points. Real-time PCR was performed to demonstrate TLR expression for specimens collected at 0, 5, 10, 20, 30 hours after autopsy (different shades of gray bars). Results from one of two experiments are shown.
• Table I - (Microsoft Word file) (45 KB).
• Table II - (Microsoft Word file) (35 KB).
• Table III - (Microsoft Word file) (30 KB).
• Table IV - (Microsoft Word file) (28 KB).