Files in this Data Supplement:

• Figure - (Microsoft PowerPoint file) (305 KB). Flow cytometry gating and analysis for peripheral blood mononuclear cells stained (PBMCs) with APC-CD133, PE-Cy7-CD34, PE-VEGFR2 and 7AAD. Forward scatter/side scatter (FSC/SSC) flow cytometric plot of the PBMCs is shown in (A). Viable cells are identified by gating on FSC/SSC plot as the 7AAD-negative population (B). CD133 positive cells (upper portion) are gated on viable cells (C). EPCs are identified by double positivity for CD34/CD133 (upper right quadrant, D). Gating and analysis for CD133/CD34/VEGFR2 triple positive cells are shown in (E). Triple positive cells are located in the upper right quadrant.
• Methods and Tables - (Microsoft Word file) (83 KB).