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• Figure I - (Adobe Acrobat file) (103 KB). Hematoxylin-eosin and Elastica van Gieson stainings and immuostainings for tenomodulin (TEM) and VEGF-A in murine heart. Analysis of serial sections showed that tenomodulin was restricted to the extracellular matrix of CTC, but not in the myocardial layer (papillary muscle) or cardiac valves. In contrast, VEGF-A expression was restricted to cardiomyocytes, but not in the cardiac valves or CTC. MV: mitral valve, PM: papillary muscle.
• Figure II - (Adobe Acrobat file) (55 KB). The CTC interstitial cells were treated with several stimuli and RT-PCR for the tenomodulin gene was performed. (A) Effect of mechanical stretching (amplitude 25%) on tenomodulin expression in CTC interstitial cells. After 6 hr, tenomodulin expression was not detected. (B) Effect of hypoxia on tenomodulin expression in CTC interstitial cells. (C) Effect of antimycin A, which mediates oxidative stress, on tenomodulin expression in CTC interstitial cells. The expression of tenomodulin was detected at a concentration of antimycin A less than 10 µg/ml, but was undetectable at 30 µg/ml antimycin A.