Files in this Data Supplement:

• Table I - (Microsoft Word) (141 kb) Sequences of oligonucleotides used for RT-PCR and real-time PCR including corresponding gene information and primer position as well as applied PCR conditions.
• Figure I - (TIF) (2.9 MB) Morphology of iPS (A, B) and ES cell (C, D) cultures during EB-based differentiation. (A, C) Day 5 floating EBs. (B, D) Outgrowth of plated EBs with contracting areas on day 24 of differentiation. Scale bars: 100 μm. Abbreviations: EBs, embryoid bodies; ES cell, embryonic stem cell; iPS cell, induced pluripotent stem cell.
• Figure II - (zipped TIF file) (742 kb) Quantification of Oct 3/4 transgene, c-Myc endogene and c-Myc transgene expression levels in iPS and ES cells on days 0, 10 and 21 of differentiation by real time RT-PCR. The mean ±SEM of normalized Oct 3/4 transgene, c-Myc endogene and c-Myc transgene expression (n=3) is depicted. *P<0.05. Abbreviations: c-Myc, cellular myelocytomatosis oncogene, ES cells, embryonic stem cells; iPS cells, induced pluripotent stem cells; Oct 3/4, Octamer binding transcription factor 3/4.
• Movie I - (Windows Media Video File) (4.22 MB) Spontaneously contracting area in an iPS cell derived EB on day 24 of differentiation.
• Movie II - (Windows Media Video File) (8.51 MB) iPS derived gut-like structure with smooth contractions indicate in vitro formation of smooth muscle cells.
• Movie III - (Windows Media Video File) (5.37 MB) Synchronous Ca²⁺ transients in clusters of iPS cell derived CMs indicating electrical coupling of the cells. Intracellular Ca²⁺ signals were recorded after incubating cells with 10 μm fluo-4 acetoxymethylester for 15 min on a laser scanning confocal microscope.
• Movie IV - (Windows Media Video File) (1.83 MB) Intracellular Ca²⁺ measurements performed on a single cell level showed homogeneous intracellular Ca²⁺ fluctuations.