P-Selectin Glycoprotein Ligand-1 Is Highly Expressed on Ly-6Chi Monocytes and a Major Determinant for Ly-6Chi Monocyte Recruitment to Sites of Atherosclerosis in Mice
Circulation An et al.
117: 3227
Data Supplement
Files in this Data Supplement:
- Figure I
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(TIF file) (2 MB). There is no significant difference in FVTII and C2GlcNAcT-1 expression between Ly-6Chi and Ly-6Clo monocytes. Real-time PCR quantification of FVTII and C2GlcNAcT-I transcripts in Ly-6Chi and Ly-6Clo monocytes (n = 9 mice). Data are means ± SEM.
- Figure II
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(TIF file) (7.3 MB). Ly-6Chi but not Ly-6Clo monocytes roll on 2-GSP-6 under flow. A. Comparison of Ly-6Chi and Ly-6Clo monocytes rolling on 2-GSP-6 at 1.0 dyn/cm2 with and without the presence of 4RA10, a mAb that blocks PSGL-1 function. B. Ly-6Chi and Ly-6Clo monocytes rolling under different shear stress. Shown are the mean ± SEM of three independent experiments.
- Figure III
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(TIf file) (3.5 MB). ApoE–/– and ApoE–/–/PSGL-1–/– mice have similar plasma lipid levels. Plasma cholesterol and triglyceride concentrations of ApoE–/– and ApoE–/–/PSGL-1–/– mice that were fasted overnight after fed the high fat diet for 12 weeks (mean ± SEM, P > 0.05, n = 14 mice). Cholesterol was measured using a kit from Wako Diagnostics, Richmond, VA, while triglyceride was measured using reagents from Sigma, according to the manufacturers’ instructions.
- Methods
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(Microsoft Office Word file) (97 KB).