Files in this Data Supplement:

• Figure I - (Microsoft PowerPoint file) (272 KB). A, A representative AP recorded at 1 Hz from a freshly-isolated cardiomyocyte before dofetilide exposure. B, An AP from the same cell in the presence of 5 nmol/L dofetilide. C, Mean±SEM APD90 before and after dofetilide (n=11 cells). **P<0.01 vs pre-dofetilide (paired t-test).
• Figure II - (Microsoft PowerPoint file) (83 KB). A, I_Ks activation and deactivation time constants (Taus) from CTL and Dof cells. Both activation and deactivation time constants were obtained by biexponetial fits to currents recorded with the voltage protocol shown at the top. B, I_Kr activation and deactivation time constants (Taus) from CTL and Dof cells. Both activation and deactivation time constants were obtained by biexponetial fit to currents recorded with the voltage protocol shown at the top. (Statistical-analysis by nonpaired t-tests. No significant differences.)
• Figure III - (Microsoft PowerPoint file) (216 KB). A, Representative examples of Late-INa from CTL (dark) and Dof (grey) cells, recorded with a 2-s pulse to a test potential of -30 mV from a holding potential of -120 mV. B, Mean±SEM Late-INa densities at -30 mV from CTL and Dof cells. (Statistical-analysis in B by nonpaired t-test. No significant differences)
• Figure IV - (Microsoft PowerPoint file) (329 KB). A&B, Representative AP recordings (1 Hz) from control (A) and dofetilide-cultured (B) cells in the absence (Before) and presence (After) of 100-nmol/L HMR-1556. C, Percentage-APD90 increase by HMR-1556 (100-nmol/L) in control and dofetilide-cultured cells (n=5 cells/group) at 1 Hz. (*P<0.05 vs. CTL, nonpaired t-test).
• Figure V - (Microsoft PowerPoint file) (30 KB). Dog 3’UTR complete sequence for KCNQ1. The full-length 3’UTR for dog KCNQ1 is 919 bp. The start site of 3’UTR is counted as “+1”. Potential miR-133 binding sites are indicated. Letters in bold indicate complementary nucleotides.
• Figure VI - (Microsoft PowerPoint file) (21 KB). Dog 3’UTR complete sequence for KCNE1. The full-length 3’UTR for dog KCNE1 is 303 bp. The start site of 3’UTR is counted as “+1”. Potential miR-1 binding site is indicated. Letters in bold indicate complementary nucleotides.
• Methods - (Microsoft Word file) (47 KB).
• Table - (Microsoft Word file) (23 KB).