Microsomal Prostaglandin E2 Synthase-1 Deletion Leads to Adverse Left Ventricular Remodeling After Myocardial Infarction
Circulation Degousee et al.
117: 1701
Data Supplement
Files in this Data Supplement:
- Methods -
(Adobe Acrobat file) (68 KB).
- Table -
(Adobe Acrobat file) (24 KB).
- Figure II -
(Adobe Acrobat file) (504 KB). Immunohistochemical analysis of COX-2 and mPGES-
1 in the LV remote from the infarct. (A) The specificity of the anti-mPGES-1 antiserum
was evaluated by staining spleen from Ptges+/+ and Ptges-/- mice. Immunohistochemical
analysis of the heart for (B) COX-2 and (C) mPGES-1 in Ptges+/+ mice, (D) COX-2 and
(E) mPGES-1 in Ptges-/- mice 0, 3, 7 and 28 days after MI. Images are representative of 3
independent experiments at each time point shown.
- Figure I
-
(TIF file) (7 MB). mPGES-1 does not modulate some signaling cascades
that regulate myocardial hypertrophy. Immunoblot analysis of (A) phospho-AKT, (B)
total AKT, (C) phospho- GSK-β, (D) total GSK-β, (E) phospho-JNK1, (F) total JNK1,
(G) phospho-PKC-ε and phospho-PKC-α (H) total PKC-ε (I) total PKC-α. Real-time
quantitative PCR analysis of (J) Nab1 mRNA. Densitometric analysis of the ratio of
phosphorylated to total protein is presented adjacent to each of the immunoblots. Data are
presented as mean±SEM of ≥5 independent experiments for each group.
- Figure III
-
(TIF file) (7 MB). Prostaglandin levels and expression of prostaglandin
biosynthetic enzymes in the LV remote from the infarct. Analysis of (A) PGD2, (B)
TXB2, (C) PGF2α and (D) 6k-PGF1α levels in the LV at 0, 3, 7 and 28 days after MI in
2
Ptges+/+ (open bars) and Ptges-/- mice (closed bars) by HPLC/ESI-MS analysis. Analysis
of (E) COX-1, (F) cPGES, (G) mPGES-2, (H) PGDS hematopoetic, (I) TXAS and (J)
PGIS mRNA in the LV remote from the infarct in Ptges+/+ (open bars) and Ptges-/- mice
(closed bars) by quantitative real time PCR. Results are normalized to GAPDH mRNA
levels. Data are presented as mean±SEM and represent ≥6 independent experiments
for each group. a, p <0.05 vs time = 0; b, p <0.05 Ptges-/- vs Ptges+/+ mice at any time
point.
- Figure IV
-
(TIF file) (7 MB). Prostaglandin levels and expression of prostaglandin
biosynthetic enzymes in the infarct and peri-infarct tissue. Analysis of (A) PGD2, (B)
TXB2, (C) PGF2‘ and (D) 6k-PGF1‘ levels in the LV at 0, 3, 7 and 28 days after MI in
Ptges+/+ (open bars) and Ptges-/- mice (closed bars) by HPLC/ESI-MS analysis. Analysis
of (E) COX-1, (F) cPGES, (G) mPGES-2, (H) PGDS hematopoetic, (I) TXAS and (J)
PGIS mRNA in Ptges+/+ (open bars) and Ptges-/- mice (closed bars) by quantitative real
time PCR. Results are normalized to GAPDH mRNA levels. Data are presented as mean±SEM and represent ≥6 independent experiments for each group. a, p <0.05 vs time =
0; b, p < 0.05 Ptges-/- vs Ptges+/+ mice at any time point.
