Cardiomyocyte Overexpression of Neuronal Nitric Oxide Synthase Delays Transition Toward Heart Failure in Response to Pressure Overload by Preserving Calcium Cycling
Circulation Loyer et al.
117: 3187
Data Supplement
Files in this Data Supplement:
- Figure I
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(Microsoft PowerPoint file) (37 KB). Left ventricle hypertrophic growth response to 14 days of isoproterenol in Ctrl and DT mice. Mean data for HW/TL ratio and summary quantification of cardiomyocyte cross-sectional area (n = 8 per group). *P<0.05 versus basal.
- Figure II
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(Microsoft PowerPoint file) (253 KB). (A) Immunoblots showing the response of calcineurin and GSK-3β (calculated by the ratio P-GSK-3β/total GSK-3β) to TAC in both genotypes and quantification results (n = 5-8 per group). TAC induced a further increase in levels of calcineurin and P-GSK-3β in DT as compared to Ctrl hearts. (B) Cardiac protein extracts were immunoprecipitated with calmodulin antibody and Western blotted to quantify coprecipitated calcineurin. *P<0.05 versus basal; †P<0.05 versus Ctrl-TAC.
- Methods
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(Microsoft Word file) (27 KB).
